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CGM Test-Stem Cells| Questions solved 100% Correct/Verified Solutions
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CGM Test-Stem Cells| Questions solved 100% Correct/Verified Solutions
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CGM Test-Stem Cells| Questions solved100% Correct/Verified Solutions
What comprises a niche? - ANSWER constraints of the space
cellular components: stromal supporting cells, descendent/progenitor cells
acellular components: soluble and membrane bound molecules
paracrine and endocrine signals
neural input
What is a niche? - ANSWER restricted and specialized tissue microenvironment that
integrates local and systemic signals for the regulation and maintenance of resident
stem cells
What is needed to culture human stem cells in vitro? - ANSWER embryonic stem
cells from the inner cell mass of a blastocyst
B fibroblast growth factor (inhibits differentiation)
mouse fibroblast feeder cells (which provide growth and attachment factors)
Fluorescence activated cell sorting (FACS) - ANSWER process used to isolate stem cells
- suspension of fluorescently tagged cells sent under pressure thru narrow
nozzle -laser
-electric field
fluorescent cells: negatively charged and can be separated
Markers commonly used to identify stem cells - ANSWER Stage-specific embryonic
antigen 3 (Embryonic stem cell and embryonal carcinoma)
, CD4 and CD8 (WBC)
Stro-1 antigen (stromal, precursor cells and hematopoietic cells)
How can you stimulate differentiation in adult and embryonic stem cells? - ANSWER
- change chemical composition of the culture medium
-alter the surface of the culture dish
-modify cells by inserting specific genes
What is regenerative medicine? - ANSWER the use of stem cells to repair or
replace damaged tissues
uses stem cells and scaffolds
*often used synonymously with tissue engineering; but regenerative medicine
places more emphasis on use of stem cells
What is a scaffold? - ANSWER biodegradable, functions like a skeleton for the organ
replaces extracellular matrix that gives body tissues their structure; consists of
tissues such as cartilage and muscle
What is tissue engineering? - ANSWER use of cells, engineering and chemical
methods to improve or replace the body's natural functions
Applications of tissue engineering - ANSWER burn victim skin grafts
orthopedic
respiratory
ophthalmologic
vascular
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