Mycobacteria marinum - Correct Answer Swimming pool granuloma
-Grows at 30°C
Mycobacteria ulcerans - Correct Answer -Painless ulcers
-Grows at 30°C
Mycobacteria fortuitum-chelonae - Correct Answer -Rapid grower
-Wound infections
-Grows at 30°C and 37°C
Mycobacteria fortuitum-chelonae biochemical tests - Correct Answer Arylsulfatase +
Growth on special MAC
Mycobacteria haemophilum - Correct Answer Skin infections, may disseminate in AIDS
patients
-Grows at 30°C
Mycobacteria haemophilum growth requirments - Correct Answer -Requires hemin,
hemoglobin or ferric ammonium citrate to grow
-Will not grow on routine media
Conventional culture methods for mycobacteria - Correct Answer Temperature - 35-
37°C (most mycobacteria prefer this temperature, a few do not)
Atmosphere- 5-10% CO2
Incubate in the dark
Incubate for 8 weeks
Non-selective egg-based culture media - Correct Answer -Lowenstein-Jensen (LJ)
-Petragnani
Selective egg-based media - Correct Answer -LJ-graft
-Mycobactosel-LJ
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