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ASCP Histology Board Study Group Prep Exam Questions Well Answered. $9.79   Add to cart

Exam (elaborations)

ASCP Histology Board Study Group Prep Exam Questions Well Answered.

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  • Course
  • Histotechnology
  • Institution
  • Histotechnology

What fixatives are preferred for staining Nuclei? Carnoy Zenkers 10% NBF Acetic Acid Bouins Alcoholic Formalin - Answer Carnoy and Acetic Acid After formalin fixation, nuclei often show coalescence of the __________ into strands with intervening clear spaces. - Answer Chromatin ...

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  • September 4, 2024
  • 8
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • Histotechnology
  • Histotechnology
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ASCP Histology Board Study Group
Prep Exam Questions Well Answered.
What fixatives are preferred for staining Nuclei?



Carnoy

Zenkers

10% NBF

Acetic Acid

Bouins

Alcoholic Formalin - Answer Carnoy and Acetic Acid



After formalin fixation, nuclei often show coalescence of the __________ into strands with intervening
clear spaces. - Answer Chromatin



What are the 2 chemicals that fix lipids? - Answer Chromic Acid and Osmium Tetroxide



What temperature does formaldehyde start to react with RNA? - Answer 45C



What temperature does formaldehyde start to react with DNA? - Answer 65C



Why doesn't glycogen get washed away in processing? - Answer Glycogen would get washed away in
processing if it weren't for the fixed proteins trapping it in the tissue



If immunoflourescence is desired, what fixative must be used?



10% NBF

Bouins

Zamboni

, Frozen, not fixed

Zenker - Answer Frozen; Not fixed



Define Osmolality - Answer Defined as the number of particles in a solution vs the tissue



What happens to the fixative if the volume of the tissue is greater than the volume of the fixative? -
Answer If the volume of the tissue is greater than the fixative, the fixative composition can be altered
due to too much of the fixative combining with tissue proteins and altering the chemical makeup of the
fixative



At what temperature can negatively affect tissue morphology? - Answer 45C



What is the approximate pH of tissue fluids? - Answer 7.2-7.4



What type of fixative must be used for a disease with urate crystals?



Alcohols

Zamboni

Frozen, not fixed

10% NBF

Zenker - Answer Alcohols



What is the pH that Electron Microscopy be done at? - Answer 7.2-7.4



What pH may alter the tissue significantly?



4

5

6

7

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