BIOL 2420 Lab Exam 1 Questions With Revised Answers
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Course
BIOL 2420
Institution
BIOL 2420
BIOL 2420 Lab Exam 1 Questions With
Revised Answers
Proper Hand washing protocols - answer1. remove any large jewelry
2. wet you hands with clean water and soap
3. rub hands together thoroughly
4. scrub for about 20 seconds
5. rinse your hands
6. dry with paper towel
spill protocols - answe...
BIOL 2420 Lab Exam 1 Questions With
Revised Answers
Proper Hand washing protocols - answer✔✔1. remove any large jewelry
2. wet you hands with clean water and soap
3. rub hands together thoroughly
4. scrub for about 20 seconds
5. rinse your hands
6. dry with paper towel
spill protocols - answer✔✔alert professor, spray a paper towel with lysol, cover contaminated
area with disinfectant soaked paper towel, leave for about 10 minutes use paper towel to wipe up
spill, dispose in the biohazard container as well as gloves
what equipment is used to achieve asepsis? - answer✔✔the inoculating loop and incinerator
medias - answer✔✔solid or liquid
slant, deep, and broth
how to label media - answer✔✔1. initials
2. date of inoculation
3. name of medium
4. the inoculum
What is the single most important way to reduce the spread of unwanted microbes? -
answer✔✔Hand washing
Where and how should used media (culture tubes and plates) be disposed of? -
answer✔✔directly into the biohazard bin
where and how should a slide prepared by you (ex. gram stain) be disposed of? -
answer✔✔Wrapped in a paper towel and disposed into the biohazard bin
Where should gloves worn by you (after lan, not cleaning up a spill) be disposed of? -
answer✔✔In the regular trash can
Where should a broken stock slide (ex. 3 forms slide that was observed during lab 3) be disposed
of? - answer✔✔In the broken glass container
What are the last two things that you always do before leaving the microbiology lab that will
ensure that you do not spread organisms used in the lab out of the lab and will also ensure that
the next class coming into the lab will not be contaminated? - answer✔✔Wash hands
disinfect lab bench
say goodbye to your classmates
put away any supplies that you used in lab
put away your lab coat
select all required information needed to properly label your cultures when labeling culture
media. - answer✔✔length of incubation
your class section
the date you plan to remove the culture
the name of the medium
your initials
the date of inoculation
the inoculum
Inoculate - answer✔✔introduce cells or organisms into a culture medium
Inoculum - answer✔✔organism or material used for inoculation
Incubator - answer✔✔equipment used to grow microorganisms in a controlled environment
Liquid media - answer✔✔Media that does not contain agar
incinerator - answer✔✔equipment used to sterilize the inoculating loops via incineration
solid media - answer✔✔media that contains the solidifying agent, agar, in it
why is it incorrect to label a culture tube cap or the lid of the petri dish? - answer✔✔lids can
easily fall off or be switched
pate media is incubated upside down
You inoculated a broth with E. coli in lab but did so at the end of the week. You won't be back in
lab until after the weekend. Therefore, the growth needs to be slowed down as the incubation
time will be longer than 72 hours. - answer✔✔25 degrees celsius incubation
You have observed your results but want to store your culture for future observations. -
answer✔✔4 degrees celsius refrigeration
Ubiquitous microorganisms - answer✔✔found everywhere
1. their small size allows for easy dissemination by air and water
2. microbes demonstrate an incredible versatility and flexibility with regards to their metabolism
3. microbes have a unique ability to tolerate extreme or unusual environments
Microorganisms are found everywhere on body surfaces and lining lumens. What beneficial
roles do microorganisms play as microbiota? - answer✔✔causes shedding of skin
aid in digestion
synthesize vitamins K and B12
synthesize vitamin C
exclude pathogens from colonizing
stimulate immune response
Why is it important as a beginning microbiologist to appreciate ubiquity of microorganisms
when working in a microbiology lab and trying to maintain aseptic technique? - answer✔✔to be
aware that microorganisms are everywhere and can very easily contaminate your cultures
Proper handling of the microscope - answer✔✔hold with one hand supporting the base and the
other holding the arm of the microscope, return it to storage cabinet
Why is oil used with the 100X objective? - answer✔✔oil helps reduce the refraction of light
how to calculate total magnification - answer✔✔ocular magnification x objective magnification
how to calculate resolving power? - answer✔✔upside y divided by (2xNA objective lens)
Select the steps you need to follow to properly handle the microscope power cord when you are
finished - answer✔✔unplug from outlet by pulling on the cord
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