MIP 302 Final Test Questions with All Correct Answers
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Course
MIP 302
Institution
MIP 302
MIP 302 Final Test Questions with All Correct Answers
What can the API test strip identify? - Answer-Gram negative rods
Why is water placed in the bottom of the API test strip? - Answer-Because the media is small it will dry up making tests hard to decipher
What does an underlined well me...
MIP 302 Final Test Questions with
All Correct Answers
What can the API test strip identify? - Answer-Gram negative rods
Why is water placed in the bottom of the API test strip? - Answer-Because the media is
small it will dry up making tests hard to decipher
What does an underlined well mean on an API strip? - Answer-Fill tube and add oil
What does a boxed well mean on an API strip? - Answer-Fill Tube and cupule with
bacteria
What does a well that is not underlined or boxed mean? - Answer-Fill just the tube
Where in API codebook should you look if Oxidase (-)? - Answer-In white section
Where in API codebook should you look if Oxidase (+)? - Answer-In blue section
When is bacteria Oxidase (+)? - Answer-If isolate is gram negative rod that does not
ferment glucose
When is bacteria Oxidase (-)? - Answer-If gram negative rod does ferment glucose
What is direct Elisa testing for? - Answer-Particular antigen
What is indirect Elisa testing for? - Answer-Antibody
Positive ELISA result - Answer-Color in well
Negative ELISA result - Answer-Colorless well
Titer - Answer-Dilution x volume used
-Reciprical of that answer
Why is blocking important? - Answer-To ensure clean results so that non specific
binding can not occur which would give a false positive result
3 types of selective media - Answer-PEA agars, MacConkey agar, and Simmons citrate
agar slants
, Component of PEA agar? - Answer-0.25% Phenyl Ethyl Alcohol
antigen capture ELISA - Answer-antibody directed against a target antigen is coated
directly onto a solid matrix. Binds with any antigen present in the sample. Used to detect
presence of a particular antigen but also used to detect presence of antibody directed
against a particular antigen.
antibody capture ELISA - Answer-directed against a particular antibody type, is coated
directly onto a solid matrix. Second antibody becomes sandwiched between the capture
antibody and the antigen. Captured by the antibody bound to the solid matrix.
Indirect elisa - Answer-detect presence of specific antibody directed against a particular
antigen, or presence of a particular antigen, enzyme will only be present to react with
substrate if the secondary antibody that it is attached to which is also attached to the
primary antibody
direct elisa - Answer-detect presence of particular antigen in a sample, antibody
carrying reporter enzyme is bound directly to the target antigen
Purpose of Blood Agar - Answer-See if bacteria has hemolysis abilities
Purpose of Catalase Test - Answer-Determine if bacteria produces catalase
Purpose of Indole Test - Answer-See if bacteria produces tryptophanase
Medium for Indole test - Answer-Tryptone broth
Purpose of Klinglers Iron Agar - Answer-See ability of bacteria to ferment glucose
and/or lactose and the ability to produce CO2 and H2S gases
Purpose of Lysine Decarboxylase Broth - Answer-Determine if bacteria produces Lysine
Decarboxylase
Purpose of MacConkey Agar - Answer-See if bacteria is gram negative and can ferment
lactose
Purpose of Methyl Red test - Answer-See if bacteria ferments glucose and produces
mixed acids
Medium from Methyl Red test - Answer-MRVP broth
Purpose of Nitrate Broth - Answer-Distinguish bacteria based on their ability to reduce
nitrate to nitrite
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