100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Previously searched by you
Previously searched by you
logo
MIP 302 Exam 1 Questions with All Correct Answers $11.99
Add to cart
Quickly navigate to
Preview
  2.  
  3. MIP 302
  4.  
  5. MIP 302

Exam (elaborations)

MIP 302 Exam 1 Questions with All Correct Answers

 0 purchase
  • Course
  • MIP 302
  • Institution
  • MIP 302

MIP 302 Exam 1 Questions with All Correct Answers What is viral cultivation? - Answer-The replication of a virus in living cells What is the main necessary component of viral cultivation? - Answer-Living cells Describe the setup of the plaque assay. - Answer-E. coli is mixed with a bacter...

[Show more]

Preview 2 out of 11  pages

Document information

  • September 4, 2024
  • 11
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

Subjects

Written for

  • MIP 302
  • MIP 302

Seller

avatar-seller
Scholarsstudyguide

Reviews received

Content preview

MIP 302 Exam 1 Questions with All
Correct Answers
What is viral cultivation? - Answer-The replication of a virus in living cells

What is the main necessary component of viral cultivation? - Answer-Living cells

Describe the setup of the plaque assay. - Answer-E. coli is mixed with a bacteriophage
in a TSA then poured into a plate; the E. coli form a bacterial lawn then the phages
infect the E. coli cells and lyse them, forming the plaques

Why did you dilute the phage stock in the plaque assay? - Answer-To calculate how
many PFUs (phages) were present on different TSA plates

Why did you add a specific volume of bacteria to each agar deep in the plaque assay? -
Answer-To keep a constant amount of bacteria in each agar deep

Why didn't you add bacteria until we were nearly ready to pour the agar deep over the
plate in the plaque assay? - Answer-We added bacteria first because the
bacteriophages need a living cell to grow in; soft agar deeps solidify quickly

How and where does the bacteria grow in the plaque assay? - Answer-The bacteria
grow within the agar overlay and form a confluent bacterial lawn

What is a plaque? - Answer-Holes formed in the lawn of bacterial cells when enough
have been infected and lysed by the phage

How is a plaque formed? - Answer-Phages infect and lyse the bacteria causing holes in
the bacterial lawn

What is a plaque forming unit? - Answer-Each plaque that is the result of an infection by
one phage

What is the dilution ratio? - Answer-# sample parts/(# sample parts + # dilute parts)

What is the formula for calculating PFUs? - Answer-PFUs in diluted sample = (PFU/mL
original sample)x(dilution)x(volume used)

What is a serial dilution ? - Answer-Stepwise dilution of a substance

What is a chemotherapeutic agent? - Answer-Agents that are administered internally to
the host for the control of microbial infections

, What is selective toxicity? - Answer-Chemotherapeutic agents must be more toxic to the
pathogen than to the host

What are some of the main mechanisms of chemotherapeutic agents? (x5) - Answer-
Inhibition of cell wall synthesis; Inhibition of protein synthesis; Inhibition of nucleic acid
synthesis; Cell membrane disruption; Metabolic antagonism

In general, how do penicillin and other B-lactam antibiotics work? - Answer-By inhibiting
cell wall synthesis

What cell types are penicillin and B-lactam antibiotics most effective against? - Answer-
Bacteria with a peptidoglycan cell wall

Why are penicillin and B-lactam antibiotics not harmful to humans? - Answer-Human
cells do not have a peptidoglycan cell wall

How does tetracycline work? - Answer-Inhibition of protein synthesis by attacking the
30s ribosome subunit

Why are eukaryotic organisms less affected by tetracycline compared to bacteria? -
Answer-Eukaryotic organisms have a 40s ribosome subunit while bacterial cells have a
30s ribosome subunit

All of the aspects of the Kirby-Bauer test are standardized in such a manner that the
only variable being tested in the bacteria's interaction with the antibiotics. How did we
standardize this experiment? - Answer-The inoculum is standardized using McFarland
Standards

What is a McFarland standard? - Answer-An inoculum with a certain density of E. coli
that the inoculum being tested can be compared to in order to ensure that the same
number of bacteria are in each sample

What are the 5 kingdoms? - Answer-Animalia, Plantae, Fungi, Protista, Monera

What kingdom(s) contain prokaryotes? - Answer-Monera

What kingdom(s) contain eukaryotes? - Answer-Animalia, Plantae, Fungi, Protista

What kingdom(s) contain microorganisms? - Answer-Protista, Fungi, Monera

What kingdom do bacteria fall into? - Answer-Monera

What kingdom do fungi fall into? - Answer-Fungi

What kingdom do protists fall into? - Answer-Protista

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller Scholarsstudyguide. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $11.99. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

69052 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 15 years now

Start selling
$11.99
  • (0)
Add to cart
Added