MIP 302 Exam 1 Questions with Answers All Correct
What does a broth being turbid represent? - Answer-bacterial growth within broth
Why would you incubate the TSB inoculated with normal flora at 37 degrees? (37 degrees Celsius is equal to 98.6 Fahrenheit) - Answer-37 degrees Celsius is the temp...
MIP 302 Exam 1 Questions with
Answers All Correct
What does a broth being turbid represent? - Answer-bacterial growth within broth
Why would you incubate the TSB inoculated with normal flora at 37 degrees? (37
degrees Celsius is equal to 98.6 Fahrenheit) - Answer-37 degrees Celsius is the
temperature of human body, therefore this mimics normal conditions for the normal flora
Why do invert a plate before incubating it? - Answer-so no condensation from lid drops
into bacteria in the hot environment of the incubator
Where do you write your name and section number on the plate? why? - Answer-on the
bottom so that the plate can still be identified even if it is separated from the lid
Why is broth not used as an isolation medium? - Answer-the different types of bacteria
that grew cannot be separated or identified
Would you use a TSA slant as an isolation medium? - Answer-no, the slant does not
allow enough surface area for growth or enough access
Why is it important that the TSA slant be inoculated with a pure bacterial culture, instead
of a mixed culture? - Answer-pure bacterial cultures must be used in order to properly
identify the identity of the bacteria in the sample
Gram stain procedure - Answer-1: primary stain - crystal violet (purple)
2: mordant- grams iodine (binds to crystal violet, still purple)
3: decolorizer- alcohol/acetone mixture (removed iodine from gram negative, gram + is
purple, gram - is colorless)
4: counterstain - safranin (turns gram - cells pink)
gram + appear what color - Answer-purple
gram - appear what color - Answer-pink
acid-fast staining procedure - Answer-1: primary stain - carbon fuchsin + heat (all cells
hot pink/red)
2: decolorized- acid alcohol mix (acid-fast + are pink, acid-fast - are colorless)
3: secondary stain - methylene blue (acid-fast - are blue)
endospores stain what color - Answer-green
vegetative cells stain what color - Answer-pink
, acid-fast + appear what color - Answer-pink/red
acid-fast - appear what color - Answer-blue
endospore staining procedure - Answer-1: primary stain - malachite green + heat (all
green at this point)
2: decolorizer - water (washes off excess stain out of vegetative cells, endospores =
green, vegetative cell = colorless)
3: secondary stain - safranin (endospores = green, vegetative cells = pink)
why do gram positive cells stain purple, and gram negative cells stain pink? - Answer-
gram + cells stain purple because they have a thick peptidoglycan layer that is too thick
for the decolorizer to remove the grams iodine, whereas gram - cells have a thin ppg
that allow for the removal of the mordant and for them to stain pink
What should you do if you get a skin cut in the lab? - Answer-Report to instructor
what should you do if you drop a test tube that contains bacterial cultures and it shatters
on the floor? - Answer-report to instructor & dispose of waste in the manner prescribed
by instructor
when should you mouth pipette? - Answer-never
list 3 items that should be discarded in the red incinerator bin - Answer-plastic tubes,
glass slides, swabs, gloves
when should you decontaminate your bench with disinfectant - Answer-when you enter
and complete lab
when is it okay to eat/drink in lab - Answer-never
what is "proper lab attire" - Answer-lab coat, close-toed shoes, safety goggles, long hair
pulled back
how should test tubes be stored when you are using them at your bench? - Answer-in
cups or racks
what is the guest policy - Answer-guests are not allowed
what criteria did you use to determine if a cell is alga or protozoan - Answer-algae: not
moving, yellow/green color
Protozoa: darker colors, moving
why are many algae green or yellow in color - Answer-because they are photosynthetic
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