MIP 616 Exam 1 Questions and Answers All Correct
which detergent is used to disrupt protein structure for electrophoretic analysis on polyacrylamide gels - Answer-SDS
what is the best technique to localize catalase in peroxisomes - Answer-A catalase monoclonal antibody and transmission electro...
MIP 616 Exam 1 Questions
and Answers All Correct
which detergent is used to disrupt protein structure for electrophoretic analysis on
polyacrylamide gels - Answer-SDS
what is the best technique to localize catalase in peroxisomes - Answer-A catalase
monoclonal antibody and transmission electron microscopy
the purpose of treating tissue from an embryonic chick with trypsin and EDTA when
generating a primary cell culture is to - Answer-digest protein-protein interactions that
hold cells together in a tissue
which of these proteins is involved in secreting proteins out of the cell - Answer-apical
which type of drug would reduce the amount of antibody produced by hybridomas -
Answer-transcription inhibitors
The phenomenon in which a chemical absorbs light at one wavelength and emits it at a
specific longer wavelength is called - Answer-fluorescence
Which of the following could be used to visualize subcellular structure in living cells -
Answer-differential interference light microscopy
to visualize cells by immunofluorescence microscopy, the cells must be - Answer-
permeabilized
the fluroescent properties of dyes such as SNARF-1 can provide information on the -
Answer-concentration of H+ ions in specific regions of teh cell
Which of the following allows one to circumvent the theoretical resolution of the
microscope - Answer-photo-activated localization microscopy
a small tumor is excised from a patient's body. The pathologist wants to examine the
number, size and arrangement of cells within the tumor. the best technique would be to
use - Answer-bright field microscopy after fixation, sectioning and staining
Amino acid in polypeptides are covalently bound by what type of bond?
a) Peptide
b) protein
,c) ester
d) phosphodiester - Answer-peptide
An alpha helix is what kind of protein structure?
a) secondary
b) primary
c) tertiary
d) quaternary - Answer-secondary
If you disrupt all hydrogen bonds in a protein, what level will preserved?
a) secondary
b) primary
c) tertiary
d) quaternary - Answer-primary
Monomeric proteins do not contain a...
a) secondary
b) primary
c) tertiary
d) quaternary - Answer-quaternary
Which is not part of a zinc finger motif?
a) zinc ion
b) proline residue
c) cysteine residue
d) histidine residue - Answer-proline residue
a small molecule that binds directly to the active site of an enzyme and disrupts its
catalytic reaction is called:
a) an allosteric inhibitor
b) a competitive inhibitor
c) a noncompetitive inhibitor
d) RNAi - Answer-a competitive inhibitor
Which statement about enzymes is TRUE?
a) The catalytic site is responsible for substrate specificity.
b) Reactions catalyzed by enzymes give the products more free energy than reactions
that occur spontaneously.
c) The rate of an enzymatic reaction is always proportional to the concentration of the
substrate
d) Enzymes increase reaction rates by lowering the activation energy needed to reach
the transition state? - Answer-Enzymes increase reaction rates by lowering the
activation energy needed to reach the transition state?
Which modification marks a protein for degradation in proteasomes?
a) Phosphorylation
,b) Ubiquitinoylation
c) acetylation
d) glycosylation - Answer-ubiquitinoylation
Kinases activates or inactivate many proteins by doing what to them?
a) methylate
b) phosphorylate
c) acetylate
d) digest - Answer-phosphorylate
The conversation if inactive chymoptrypsinogen to active chymotryosin is an example
of?
a) proteolytic activation
b) positive cooperativity
c) allostery
d) Ligand induced activation - Answer-proteolytic activation
Which amino acid can be phosphorylated?
a) lysine
b) serine
c) glycine
d) proline - Answer-serine
G proteins act as...
a) a catch
b) a branch
c) a switch
d) a notch - Answer-a switch
what do g proteins do to their bound nucleotide triphosphate?
a) nebulize it
b) hydrolyze it
c) customize it
d) homogenize it - Answer-hydrolyze it
What kinds of proteins accelerate nucleotide hydrolysis by G proteins?
a) guanine nucleotide exchange factor
b) GTPase accelerating protein
c) nuclear localization signal proteins
d) nucleoproins - Answer-GTPase accelerating protein
In two-dimensional gel electrophoresis, proteins are first resolved by... and then by...
a) isoelectric focusing; SDS-PAGE
b) SDA-PAGE; affinity chromatography
c) SDS-PAGE; ion exchange
d) isoelectric focusing; gel filtration - Answer-Isoelectric focusing; SDS-PAGE
, Gel filtration chromatography separates proteins on the basis of their:
a) charge.
b) mass.
c) affinity for a ligand.
d) shape. - Answer-mass
Which detergent is used to disrupt protein structure for electrophoretic analysis on
polyacrylamide gels?
a) Tide
b) Triton X-100
c) Nonidet P40 (NP40)
d) Sodium dodecylsulfate (SDS) - Answer-sodium dodecylsulfate (SDS)
Tissue is treated to break open each cell's membrane and release the contents, and
then subjected to differential centrifugation. Which of the following is TRUE after
centrifugation?
a) Depending on centrifugation speed, proteasomes are more likely to be in the
supernatant than chaperones.
b) Proteins of similar density will be found in the same fraction (either pellet or
supernatant).
c) The pellet contains the least dense material.
d) The pellet will contain a purified protein for further analysis. - Answer-Proteins of
similar density will be found in the same fraction (either pellet or supernatant).
Proteomics allows researchers to:
a) examine where a protein is located within the cytosol
of a cell.
b) compare thousands of samples from different people in the same experiment.
c) examine which proteins differ in abundance between a normal sample and a disease
sample.
d) use antibodies to label specific proteins on an SDS-PAGE gel. - Answer-examine
which proteins differ in abundance between a normal sample and a disease sample.
What factors necessary for growth of animal cells
in culture are provided by serum?
a) amino acids
b) precursors of DNA synthesis
c) growth factors
d) vitamins - Answer-growth factors
Which amino acid can be phosphorylated?
a) lysine
b) threonine
c) glycine
d) proline - Answer-threonine
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