HTL ASCP Exam- Nuclear and Cytoplasmic Staining Exam Questions With Correct Answers.
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Course
HTL ASCP
Institution
HTL ASCP
HTL ASCP Exam- Nuclear and Cytoplasmic
Staining Exam Questions With Correct
Answers.
Heterochromatin - answerCondensed regions of chromosomes that can be seen in light
microscopes because of basophilic nuclear material, ie lymphocytes.
Euchromatin - answerGenetically active DNA that is not see...
HTL ASCP Exam- Nuclear and Cytoplasmic
Staining Exam Questions With Correct
Answers.
Heterochromatin - answer✔✔Condensed regions of chromosomes that can be seen in light
microscopes because of basophilic nuclear material, ie lymphocytes.
Euchromatin - answer✔✔Genetically active DNA that is not seen with hematoxylin staining, ie
neuronal nuclei.
Nuclear stain - answer✔✔This type of stain uses positive (cationic, basic) dyes to attach to the
negatively-charged DNA/RNA.
Aluminium, iron, chromium - answer✔✔3 example of the metallic ions involved in the hematein
metal-mordant staining technique.
Amphoteric - answer✔✔Terminal amino group and terminal carboxyl group on the other end of
the protein chains making their charge pH-dependent; this is called?
IEP - Isoelectric point of pH 6 - answer✔✔For proteins, this point is when the negative and
positive charges are equal and there is no migration of proteins to the cathode (-) or anode (+).
Below pH 6 - answer✔✔This pH makes cytoplasmic proteins develop a positive charge and
attracted to anionic dyes such as eosin.
Mordants - answer✔✔Metals or other substances that act as a link between dye and tissue.
Differentiation in regressive staining techniques. - answer✔✔Weak acid solution dip, excess
mordant solutions, oxidizer solutions are used for this reason.
Oxidation - answer✔✔Sodium iodate, mercuric chloride and potassium permanganate are used
to achieve this process also called ripening of hematoxylin.
Non-oxidizing mordants - answer✔✔Ammonium or potassium aluminum sulfate,
phosphotungstic acid and phosphomolybdic acid are used to achieve the stability of the "lake".
Acid or Aluminum - answer✔✔Excess of these substances can create more selective nuclear
staining.
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