100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Summary of Lesson 9 “iPSCs” $8.03   Add to cart

Summary

Summary of Lesson 9 “iPSCs”

 6 views  0 purchase
  • Course
  • Institution

This document includes the summary (info slides + lesson notes) of lesson 9 of the course 'genome technology and applications'. Lesson 4 was simply an introduction by NGS to the task. Here, you must give a group presentation about a certain NGS technique.

Preview 2 out of 8  pages

  • September 7, 2024
  • 8
  • 2023/2024
  • Summary
avatar-seller
Induced pluripotent stem cells (iPSCs)
1. What
Stem cells
- Can divide and create an iden-cal copy of themselves, a process called self-renewal
- Can differen-ate/mature into cells that make up every type or several types of
-ssue(s) and organ(s) in the body, a process called
pluripotency/to-potency/mul-potency
o Pluripotent: differen-ate to many other celtypes
o To-potent: differen-ate to all celtypes
o Mul-potent: selec-on of celtypes
Potency
- Different types:
o Early embryonic cells – to-potency
§ First cells created -> generated placenta and embryo-c cells
o Embryonic stem cells – pluripotency
§ AJer one week you have cells surrounding the the embryo
o Adult stem cells: e.g., hematopoie-c stem cell – mul-potency
§ Differen-ate to certain celtypes
o Induced pluripotent stem cells – pluripotency
Induced
- You start from a soma-c cell -> bring them back to a stem cell
- You add Yamanaka cells
IPSC discovery
- Different TF will bring your soma-c cells back to stem cells
- Human IPSC
o Start from fibroblast
o Couple of TF that are crucial
o It needs to be able to form 3 germ layers

2. How?
IPSC crea5on
Soma,c cells
- Start from fibroblast
- Puncture skin
o Advantages: easily to get
o Disadvantage: for some people it is s-ll to invasive and UV exposure
§ Muta-on from sun exposure is and you don’t want this
- Alterna-ves
Yamanaka factors
- 4 factors
- Different hypothesis how it is possible to go to stem cells with only 4 factors
o S-ll need to be inves-gated

, - How do you get them in
o Integrated in the genome
§ You can do it with viral methods
• + transduc-on efficiency
• + You can do it for dividing and non-dividing cells
• - Random integra-on in te genome -> disadvantage
o cMyc is a oncological driver -> can be a problem, oncological
proper-es
• - Reac-va-on of transgene
§ Non viral methods
• - Nucleofec-on/transfec-on efficiency
• - They can also re-integrate in the genome
o Non-integra-ve system
§ Viral
• It can stay quite long in the cell -> it taked quite of passages to get it out
or you can do a heat shock
• Good transduc-on efficiency
§ Non-viral
• Effect is gone quite rapidly
• Transient
• Lower reprogramming efficiency
-
Valida,on
iPSC-specific
- Morphology: visual inspec-on of colonies
o Group of cells that are iden-cal = colony
o Well defined boarder without spikes
o Cells -ghtly packed
o Ra-o of the nucleus in comparison with the cytoplasm is very high
o Nucleoli are very prominent
o Bright center in the middle

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller evagoormans. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $8.03. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

85443 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling
$8.03
  • (0)
  Add to cart