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BCH 403 – CUMULATIVE TEST QUESTIONS WITH ALL CORRECT ANSWERS

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BCH 403 – CUMULATIVE TEST QUESTIONS WITH ALL CORRECT ANSWERS During translocation, the ribosome is moved towards the 3' end of the mRNA. In eukaryotes, this requires: A. eEF1α. B. eEF1βγ. C. eEF2. D. EF-G. E. EF-Ts - Answer-C. eEF2 Peptidyltransferase: A. requires GTP as an energy s...

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  • September 7, 2024
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  • 2024/2025
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  • Questions & answers

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BCH 403 – CUMULATIVE TEST
QUESTIONS WITH ALL CORRECT
ANSWERS
During translocation, the ribosome is moved towards the 3' end of the mRNA. In
eukaryotes, this requires:
A. eEF1α.
B. eEF1βγ.
C. eEF2.
D. EF-G.
E. EF-Ts - Answer-C. eEF2

Peptidyltransferase:
A. requires GTP as an energy source.
B. is found on the large ribosomal subunit in both prokaryotes and eukaryotes.
C. is found within the protein components of the ribosome.
D. acts between the P- and E-sites of the ribosome.
E. catalyze the bond between the 3' of tRNA and a peptide. - Answer-B. is found on the
large ribosomal subunit in both prokaryotes and eukaryotes

,Proteins synthesized by rough endoplasmic reticulum (RER)-bound ribosomes are
transported to all of the following organelles, except:
A. Endoplasmic reticulum.
B. Golgi.
C. Lysosome.
D. Nucleus.
E. Extracellular. - Answer-D. Nucleus

Which one of the following riboendonucleases function in the nucleus?
A. Drosha.
B. Dicer.
C. Argonaute.
D. RNaseIII.
E. RNaseE. - Answer-A. Drosha

Which one of the following riboendonucleases will combine with miRNA to inhibit
initiation of translation?
A. Drosha.
B. Dicer.
C. Argonaute.
D. RNasIII.
E. RNaseE. - Answer-C. Argonaute

Which one of the following aspects of eukaryotic gene expression cannot be accurately
predicted using a sequenced genome?
A. Kozak sequence.
B. Transcriptional promoters.
C. Translational stops sites.
D. Protein sequence.
E. Alternative splicing. - Answer-E. Alternative splicing

A biochemical technique that can be used to identify all areas of DNA bound by
histones is called:
A. DNA-seq.
B. ChIP-seq.
C. DNase-seq.
D. RNA-seq.
E. Proteomics. - Answer-B. ChIP-seq

One advantage of artificial restriction enzymes compared to the CRISPR-Cas9 system
is:
A. Artificial restriction enzymes can interact with methylated DNA.
B. Artificial restriction enzymes rely on RNA-DNA interaction.
C. Artificial restriction enzymes have fewer off target effects.
D. Artificial restriction enzymes can be used to control transcription.
E. Artificial restriction enzymes can be used to located specific DNA sequence. -
Answer-C. Artificial restriction enzymes have fewer off target effects.

All mutations that are directly involved in the development of cancer are called:

, A. Driver mutations.
B. Passenger mutations.
C. Oncogenes.
D. Tumor suppressors.
E. Sporadic cancers. - Answer-A. Driver mutations

Which one of the following is NOT a function of tumor suppressors?
A. Acts to prevent unwanted cell proliferation.
B. Monitor DNA for damage.
C. Induce apoptosis.
D. Acts to prevent unwanted DNA replication.
E. Provide signals to enter the cell cycle - Answer-E. Provide signals to enter the cell
cycle

Which of the following mutations will never affect protein structure?
A. Missense mutation.
B. Nonsense mutation.
C. Frameshift mutation.
D. Synonymous mutation.
E. Non-synonymous mutation. - Answer-D. Synonymous mutation

Which one of the following statements regarding glucose oxidation is correct?
A. All 6 carbons from glucose are oxidized to CO2 during the TCA cycle
B. Only 2 of the carbons from glucose are oxidized to CO2, and this occurs during the
TCA cycle.
C. Only 4 of the carbons from glucose are oxidized to CO2, and this occurs during the
TCA cycle
D. 2 carbons are oxidized to CO2 during glycolysis, while 4 carbons are oxidized to
CO2 during the TCA cycle
E. 2 carbons are oxidized to CO2 by the pyruvate dehydrogenase complex, while 4
carbons are oxidized to CO2 during the TCA cycle - Answer-E. 2 carbons are oxidized
to CO2 by the pyruvate dehydrogenase complex, while 4 carbons are oxidized to CO2
during the TCA cycle

Which one of the following TCA cycle enzymes is irreversible and regulated?
A. Citrate synthase
B. Isocitrate dehydrogenase
C. Succinate dehydrogenase
D. α-ketoglutarate dehydrogenase
E. Aconitase - Answer-A. Citrate synthase

Which of the following statements is correct regarding the regulation of pyruvate
dehydrogenase complex?
A. Acetyl-CoA inhibits activity of pyruvate dehydrogenase by activating pyruvate
dehydrogenase (PDH) kinase
B. Acetyl-CoA increases activity of pyruvate dehydrogenase by activating pyruvate
dehydrogenase (PDH) kinase
C. Acetyl-CoA inhibits activity of pyruvate dehydrogenase by activating pyruvate
dehydrogenase (PDH) phosphatase
D. Acetyl-CoA increases activity of pyruvate dehydrogenase by activating pyruvate
dehydrogenase (PDH) kinase

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