BACE Pretest UF Practice
Quizzes questions and answers
Why might some people be ethically opposed to using CRISPR/Cas9
to create mutations in animal embryos? - answer Alterations in
germline cells will be passed onto future generations
Which of the following is the study of the entire set of proteins of a
cell or organism? - answer Proteomics
Which department within a biotechnology company is responsible
for document control, and for the oversight of regulatory
compliance? - answer Quality Assurance (QA)
Good Laboratory Practice (GLP) is a quality system intended to
ensure regulatory compliance in pre-clinical laboratory studies -
answer True
Phase with volunteers who have the disease or condition. The
primary focus is efficacy and determining side effects. - answer CTP:
Phase 2
Phase with volunteers who have the disease or condition. The
primary focus is a broad scale study of the drug's efficacy, and
monitoring side effects. - answer CTP: phase 3
Phase with healthy volunteers or people with the disease/condition.
The primary focus is on studying safety parameters of the drug. -
answer CTP: phase 1
, Which of the following features of bacterial plasmids can be used as
a marker when growing bacterial cultures? - answer Antibiotic
resistance gene
When using an acrylamide gel for protein electrophoresis, it is best
to use a higher percentage of acrylamide to resolve smaller
proteins, while large proteins are best resolved on low-percent gels.
- answer True
PAGE: Staining with Coomassie Blue - answer To visualize proteins
after PAGE
PAGE: Adding SDS to the sample buffer and the PAGE running buffer
- answer To coat the sample proteins with a negative charge, and
denature them
PAGE: Adding loading dye to protein samples - answer To facilitate
loading samples into wells, and to monitoring sample migration
during PAGE
Your supervisor asks you to run a PCR reaction with products
ranging in size from 200-400 base pairs. To confirm the reaction
outcome on an agarose gel, which of the following strategies will
give you the best resolution of the PCR products? - answer Use a
higher percentage of agarose and a lower running voltage
In recombinant DNA technology, DNA ligase is used to rejoin which
of the following bonds in DNA? - answer Phosphodiester
A restriction enzyme digestion reaction contains which of the
following reagents? - answer DNA, Water, Buffer, Restriction Enzyme
Isolate gene of interest - answer general protocol for genetic
engineering: step 1
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