CHT EXAM COMPLETE STUDY GUIDE Final Exam Questions And Answers Graded A
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Course
CHT Em 2023 -20
Institution
CHT Em 2023 -20
CHT EXAM COMPLETE STUDY GUIDE Final
Exam Questions And Answers
Graded A
DTT - ANSWER-dithiothreitol, also known as Cleland's
Reagent
DTT inactivates IgM molecules by cleaving the _________
in the
pentamer structure. - ANSWER-disulphide bonds
Heat inactivation works by cleaving the ...
CHT EXAM COMPLETE STUDY GUIDE Final
Exam 2023 -2024 Questions And Answers
Graded A
DTT - ANSWER-dithiothreitol, also known as Cleland's
Reagent
DTT inactivates IgM molecules by cleaving the _________
in the
pentamer structure. - ANSWER-disulphide bonds
Heat inactivation works by cleaving the disulphide bonds
in the
,pentamer structure, but is less specific than _____. -
ANSWER-DTT.
ALA - ANSWER-therapeutic antilymphocyte
antibodies
Thymoglobulin - ANSWER-purified, pasteurized, gamma
immune globulin,
obtained by immunization of rabbits with human
thymocytes
OKT3 - ANSWER-a murine IgG monoclonal antibody
directed against CD3
If these therapeutic antilymphocyte
antibodies are present in the patient serum, they can
cause_______ reactivity in
T cell cytotoxic assays. - ANSWER-false positive
,ALA removal is accomplished by absorption using
_____coated with anti-rabbit or anti-mouse
immunoglobulin for Thymoblobulin or OKT3,
respectively. - ANSWER-magnetic beads
serum preparations - ANSWER-1. Recalcificiation of
plasma
2. Adsorption with lymphocytes
3. Inactivation of IgM antibodies
a. DTT treatment
b. Heat Inactivation
4. Depletion of therapeutic anti-lymphocyte antibodies
from serum
Why is calcium added to plasma to convert it to serum? -
ANSWER-Calcium is added to the plasma to combine
with the citrate present in the anticoagulant.
This restores a normal ionized calcium level and allows
clotting to occur.
, At what temperature should serum absorption with
lymphocytes be performed? - ANSWER-Typically, auto-
antibodies are cold reacting and should be absorbed at
4oC while alloantibodies
are warm reacting and should be absorbed at 37oC.
DTT treatment is more specific than heat treatment in
removing IgM antibodies. However, DTT can also affect
the _______ in IgG antibodies. - ANSWER-disulfide bonds
What happens if we don't remove OKT3 prior to
cytotoxicity assays? - ANSWER-OKT3 is a monoclonal
antibody directed against the CD3 molecule. Therefore,
the
binding of OKT3 to any CD3+ cell will activate
complement and lyse the target cell. This results
in falsely positive reactivity.
Lymphocytes have a density of_______ - ANSWER-
~1.077 g/ml.
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