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WGU D311 MICRO SECTION 1 EXAM QUESTIONS AND ANSWERS 2024
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WGU D311 MICRO SECTION 1 EXAM QUESTIONS AND ANSWERS 2024
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WGU D311 MICRO SECTION 1 EXAMBiofilms - ANSWERS-Microorganisms grow
highly structured
complex and dynamic ecosystem
provide advantages to microorganisms they are on
Advantages of biofilms - ANSWERS-Adhesion/ cohesion, nutritional sources,
protection and resistance to drugs, host immune attacks (antibodies/complement
system)
Structure of biofilms - ANSWERS-Conditions influence structure
filamentous biofilms: rapidly flowing water
mushrooms like shape: slow moving water
EPS: large part of biofilm,maintains integrity and functions in the biofilm, allows
movt of nutrients, waste and gases, Kronos cells hydrated, protect organisms in
biofilm from predators
Planktonic cell - ANSWERS-Free floating cells that lives in water
Sessile cell - ANSWERS-Biofilm forms/attached to a surface
,quorum sensing - ANSWERS-How biofilms coordinate activities due to
environmental stimuli
What are examples of biofilms - ANSWERS-Beneficial: layers of micro iota lining
the intestinal and resp mucosa play a role in warding odd infections by pathogens
harmful: plaque that forms in teeth can contribute ti dental and periodontal
disease
Light microscopy - ANSWERS-Use light to see image
Structures of bright field microscope - ANSWERS-Commonly used
2 or more lenses produce dark image on a bright background
ocular lens
multiple objective lenses with different magnification levels.
highest magnification is 1000x magnification (100x in objective lens and 10x in
ocular lens
electron microscope - ANSWERS-A microscope that forms an image by focusing
beams of electrons onto a specimen. 1000 times more detailed than light.
Requires a vacuum so no living specimen.
What are two types of electron microscopes - ANSWERS-Transmission and
scanning electron microscopes
, transmission microscope - ANSWERS-Uses electron beam from above the
specimen that is focused on magnetic lens then projected through a specimen
onto a detector. Electrons pass through the specimen and the detector captures
the image
scanning electron microscope - ANSWERS-3D Images. Very detailed. Scan a
narrow beam of electrons back and forth.
Best microscope for biofilms - ANSWERS-Confocal microscope
wet mount - ANSWERS-Simplest type of prep needed for microscopy
specimen on slide, drop liquid/stain, coverslip, then exam under scope
Fixation - ANSWERS-Attach cells to slide by heat or chemicals.
kills microorganisms
preserves integrity of cellular components
What are characteristics of Prokaryotic Cells "Bacteria" - ANSWERS-Single celled,
do not have nucleus, mitochondria or chloroplast, multiple shapes (coccus,
bacillus, vibrio, etc) found in pairs, clusters or chains
What are characteristics of Eukaryotic cells "animal and plant cells" - ANSWERS-
Have a nucleus, have membrane bound organelles, single celled or multicellular,
larger than prokaryotes, linear histone wrapped chromosomes
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