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Cell Biology Lab Final Exam Questions with Answers
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Cell Biology Lab Final Exam Questions with Answers
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Cell Biology Lab Final Exam(A&B) Biochemical reaction consume oxygen
Biochemical reaction evolve oxygen - ANS-The oxygen electrode can be used to measure
(A&B) Name, compositition and physical/chemical properties
B: Toxicology information, Handling and deposing - ANS-What information must be present on a
MSDS
(A&B) The pKa value of acid represent the center of buffer capacity
Contains equal concentration of acid and conjugate base - ANS-The most effective buffering
system
(A&C) Adjust the temperature of the meter with the room temperature
Calibrate the electrode using standard buffers - ANS-Before measuring the pH of a solution
(A&C) Allows the metal cation (Na+) pass through the glass
Is made from a special glass, coated both sides with a layer of hydrated gel - ANS-In a pH
meter, the glass bulb at the bottom of sensing electrode
1. Calculate concentration of unknown using direct spectrophotometer
2. Identify unknown compound using absorbance spectrum
3. Measurement of color intensity to calculate concentration - ANS-NAME THREE
APPLICATION OF SPECTROPHOtoMETRIC TECHNIQUE
1. positive charge
2. neutral charge
3. negative charge - ANS-in the ions-exchange column, below, what is the order of elution of the
following compounds when you change the salt gradient of the mobile phase buffer
10 to 10^-14M - ANS-What is the range of H+ concentration in solutions
1000 - ANS-If the pH value of a soultion changes by 2 units (for example from 7 to 5), the [H+)
would change by
10000 - ANS-You wish to centrifuge and isolate cytosolic fraction of a human tissue sample at
10,000 rpm, by using a nomogram (provided). Determine at what relative centrifugation force
(xg) you have to run the sample, assuming a radius
12 grams carbon, carbon-12 - ANS-A mole is the amount of a substance that contain exactly as
many particles as there are in exactly _____________ of _________________.
, 2- serum albumin (MW: 65,000)
1- Egg white albumin (MW:45,000)
4- Chymotripsin (MW: 21,000)
3- Lysozyme (MW:13,000) - ANS-In a denaturing PAGE, in what order of the following proteins
will be resolved (from the top of the gel to bottom)
200nm - ANS-nucleic acis display intense absorption at
250 ml graduate cylinder - ANS-What measuring vessel is more appropriate to make a 200ml
solution if ONLY the following vessels are available
4 fragments - ANS-If a palindromic sequence repeats 4 times through a circular DNA, a
restriction enzyme that recognize these bases will cut DNA into
45,000 - ANS-The molecular weight of a protein which travels to 25 mm distance from the well
5 - ANS-using above column, how many distinguishable fractions you would get at the end of
fractionation
5000 - ANS-The molecular weight of a protein which travels to 65 mm distance from the well
100,000
500ml volumetric flask - ANS-What measuring vessel is more appropriate is more appropriate to
make a 500ml solution
7 fragments - ANS-If a palindromic sequence repeats 6 times through a piece of linear DNA,
restriction enzyme that recognize these bases will cut the DNA molecule
80-90% - ANS-At what confluency the cells need to be splitted
A & C - ANS-Most primary cell culture have limited lifespan unless
a and b - ANS-why you have to keep all the fractions on the ice during the fractionations steps
a and c - ANS-Centrifugation techniques are used for
a and c - ANS-in all type of chromatography, the stationary phase may be
a and c - ANS-In SDS-PAGE, the proteins are compressed at the top of resolving gel because
of:
a cation exchanger column - ANS-A resin that has negatively charged functional groups
A photon - ANS-Hydrogene ion is just
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