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GMS6504 chromatography MS Exam Questions And Correct Answers
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GMS6504 chromatography MS Exam QuestionsAnd Correct Answers
partitioning
2
chromatographic efficiency
3
classes of mass spectrometers
4
solvent strength
Terms in this set (27)
chromatography
separates compounds based on differential preference to bind to TWO PHASES
mobile phase
stationary phase
partitioning
upper phase
lower phase
basis of separation
adsorption
partition
ion-exchange chromatography
size-exclusion chromatography
electrophoresis = smaller particles move faster ?
column chromatography
, column used
solid for stationary phase
liquid for mobile phase
gravity pulls mixture down = can separate
chromatographic efficiency
ability to separate one compound from another
variables that affect resolution
-d = how far apart the peaks are
-the greater the d = the better the resolution
-w = width of the peaks = the lower w is (narrower) the better the resolution is
ways to improve resolution
-moving the peaks further apart
-make them skinnier
phase polarity
-polarity of analyte, stationary phase, and mobile phase determines retention
-reverse phase is most common = non-polar stationary phase and polar mobile phase
polarity
-intermolecular forces btwn mobile phase and sample, and stationary phase and sample
govern retention of solutes gel permeation chromatography -AKA size exclusion
chromatography -separates based on molecular size -stationary phase filled with pores
-large molecules are too large to fit so are excluded = pass through quickly -small
molecules permeate the stationary phase = pass through slowly mechanisms of
chromatography adsorption: -most common for small molecules -two types of
adsorbents = nonpolar and polar -most common are silica and alumina
ion exchange chromatography
-mobile phase is NEUTRAL
-stationary phase is CHARGED
-anion exchange = A- + R+B- --> B- + R+A-
-cation exchange = A+ + R-B+ --> B+ + R-A+
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