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UIOWA Microbiology Exam 1 Questions & 100% Correct Answers

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Mitochondria similarity to prokaryotes (bacteria) ~~> Circular DNA and 70S ribosomes 70S ribosomes subunits are ~~> 50S and 30S 70S ribosomes found in ~~> Prokaryotes (bacteria & archaea) (and mitochondria/chloroplasts) 80S ribosomes found in ~~> Eukaryotic cells (algae, fu...

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  • September 13, 2024
  • 35
  • 2024/2025
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  • Uiowa Microbiology
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1 | P a g e | © copyright 2024/2025 | Grade A+




UIOWA Microbiology Exam 1 Questions &
100% Correct Answers
Mitochondria similarity to prokaryotes (bacteria)

✓ ~~> Circular DNA and 70S ribosomes




70S ribosomes subunits are

✓ ~~> 50S and 30S




70S ribosomes found in

✓ ~~> Prokaryotes (bacteria & archaea) (and

mitochondria/chloroplasts)




80S ribosomes found in

✓ ~~> Eukaryotic cells (algae, fungi, protozoa, helminths and animals)




80S ribosomes subunits are

✓ ~~> 60S and 40S




Bacilli




Master01 | September, 2024/2025 | Latest update

, 2 | P a g e | © copyright 2024/2025 | Grade A+



✓ ~~> Rod, gram positive, endospores, aerobic or facultative anaerobes




Cocci

✓ ~~> Round, gram positive or negative, anaerobic




LPS (lipopolysaccharide)

✓ ~~> Outer layer of gram negative bacteria (contains Lipid A

endotoxin)




Gram stain steps

✓ ~~> Stain with crystal violet, iodine in place, destain with alcohol,

counterstain with safranin




Two major sugars in peptidoglycan

✓ ~~> N-acetylglucoasmine (G) and N-acetylmuramic acid (M)




Bacteria able to resist osmotic pressure due to their

✓ ~~> cell wall




Enzyme functions




Master01 | September, 2024/2025 | Latest update

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✓ ~~> Accelerate, bring substrates together, make or break molecular

bonds (does not carry out a reaction that would otherwise not

happen)




Four divisions in cell wall structure

✓ ~~> Gracilicutes, firmicutes, tenericutes, mendosicutes




Gracilicutes

✓ ~~> Thin skinned gram negative cell walls




Firmicutes

✓ ~~> Thick gram positive cell walls




Tenericutes

✓ ~~> Soft, lack cell wall




Mendosicutes

✓ ~~> No peptidoglycan (murein) in cell walls (can stain both gram + & -

) (archaea)




Apoenzyme



Master01 | September, 2024/2025 | Latest update

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✓ ~~> Protein portion of enzyme




Coenzymes vs Cofactor (chemical difference)

✓ ~~> Coenzymes usually organic (cofactors usually metals)




Obligate intracellular parasite

✓ ~~> Can't live or reproduce outside host cell (viruses)




Oxidative phosphorylation location in prokaryotes

✓ ~~> Plasma membrane




Oxidative phosphorylation location in eukaryotes

✓ ~~> Inner mitochondrial membrane




Prokaryote cell types

✓ ~~> Bacteria and archaea




Eukaryotes cell types

✓ ~~> Fungi, protazoa, helminths animals, plants




Prokaryotes have no


Master01 | September, 2024/2025 | Latest update

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