Mip 302 Final Exam Practice Questions and Answers | 100% Pass

Mip 302 Final Exam Practice Questions and Answers | 100% Pass What is an microaerophile? - Answer️️ -Require atmospheric oxygen just at low levels What is a facultative anaerobe? - Answer️️ -Grow with or without oxygen What is an obligate anaerobe? - Answer️️ -Only grown in total absence of atmospheric oxygen What condition is a Gaspeak Jar - Answer️️ -Anaerobic environment What is the redox indicator placed in the Gaspeak Jar? - Answer️️ -Methylene blue What does the catalase test, test? - Answer️️ -Catalase enzyme What is the reagent used in the catalase test? - Answer️️ -hydrogen peroxide (H2O2) What does the oxidase test test for? - Answer️️ -presence of cytochrome c oxidase What is the reagent used in the oxidase test? - Answer️️ -P-amino-dimentylaniline monohydrochloride positive oxidase test - Answer️️ -Reagent turns blue after 10-20s; because in the presence of cytochrome C oxidase, electrons are transferred from TMPD to Cytochrome C negative oxidase test - Answer️️ -colorless phenol red positive - Answer️️ -yellow (Acidic) Phenol Red Negative - Answer️️ -red (Basic) ©SOPHIABENNETT EXAM SOLUTIONS Saturday, September 7, 2024 6:58 PM Page | 2 Bacteria that ferment peptone and beef extract will turn? - Answer️️ -Hot pink Nitrate broth - Answer️️ -differential medium that distinguishes bacteria based on their ability to reduce nitrate or nitrite during anaerobic respiration- nutrient broth with a small amount of potassium nitrate Reagents: Nitrate Reduction test - Answer️️ -Nitrate A and Nitrate B Nitrate Reduction Test positive - Answer️️ -Red coloration after the addition Nitrate A and B reagents OR No color change after the addition of zinc Nitrated reductive negative - Answer️️ -Red after Zinc addition or, red apron addition of reagents Is MacConkey agar selective or differential? - Answer️️ -both selective and differential What is a Maconkey Agar selective for? - Answer️️ -Growth of Gram negative bacilli What is MacConkey agar differential for? - Answer️️ -lactose fermentation What is the pH indicator in MacConkey agar? - Answer️️ -neutral red, red in acidic environments yellow in basic environments positive lactose fermentation on MacConkey - Answer️️ -Red or hot pink colonies h Growth on MacConkey - Answer️️ -gram negative Rods Is PEA agar selective or differential? - Answer️️ -selective for growth of Gram positive Organisms What is in PEA agar that makes it selective? - Answer️️ -Phenylethy alcohol Is blood agar selective or differential? - Answer️️ -differential What does blood agar test? - Answer️️ -Hemolysis ©SOPHIABENNETT EXAM SOLUTIONS Saturday, September 7, 2024 6:58 PM Page | 3 What does beta hemolysis look like? - Answer️️ -Complete digestion of the RBC & Hb; see through the media What does alpha hemolysis look like? - Answer️️ -Partial digestion of the RBC & Hb; brownish-greenish color What does gamma hemolysis look like? - Answer️️ -No change in media Is pseudoscience agar selective or difffrential? - Answer️️ -It is both selective and differential What is Pseudosel selective for? - Answer️️ -Pseudomonas Aerugionosa What is Pseudosel Differential for? - Answer️️ -Isolation and identification of Pseudomonas aeruginosa What is in Pseudosel agar that makes it selective? - Answer️️ -Cetrimide What pigments does P. Aeruginosa produce - Answer️️ -Pyocynanin Is Pseudomonas Agar F selective or differential? - Answer️️ -It is a differential medium, based on ability to produce fluorescein. What medium is the indole test performed in? - Answer️️ -Tryptone broth What does the indole test for? - Answer️️ -presence of tryptopha