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bioc LAT2 Practice Questions and Answers | 100% Pass Select the BEST answer. The percentage oxyhaemoglobin in a haemoglobin solution can be calculated by: using the ratio of absorbances of oxy- and deoxyhaemoglobin at wavelengths where the biggest difference in absorbance readings are observed...

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  • September 13, 2024
  • 18
  • 2024/2025
  • Exam (elaborations)
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  • bioc lat2 practice questions and answers 100 pa

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©SOPHIABENNETT
EXAM_SOLUTIONS GUARANTEED SUCCESS 2024/2025 ACADEMIC YEAR




©SOPHIABENNETT 9/3/24 2024/2025

, ©SOPHIABENNETT EXAM SOLUTIONS_2024/2025 Tuesday, September 3, 2024 10:30 AM



bioc LAT2 Practice Questions and
Answers | 100% Pass
Select the BEST answer.

The percentage oxyhaemoglobin in a haemoglobin solution can be calculated by:



using the ratio of absorbances of oxy- and deoxyhaemoglobin at wavelengths where the

biggest difference in absorbance readings are observed between the two forms.



adding a live yeast suspension to an oxyhaemoglobin solution and observe the change

in absorbance by spectrophotometry.



measuring the absorbance of an oxyhaemoglobin solution using the wavelength that

corresponds to its absorption maxima and then apply the Beer-Lambert formula.



measuring the absorbance of a haemoglobin solition at 560 nm, which is

deoxyhaemoglobin's absorption maxima and the wavelenth where oxyhaemoglobin

absorb light the weakest. - Answer✔️✔️-using the ratio of absorbances of oxy- and

deoxyhaemoglobin at wavelengths where the biggest difference in absorbance readings

are observed between the two forms.

Absorbance may be defined as:



the ratio of lightpath length to incident light


Page | 1

, ©SOPHIABENNETT EXAM SOLUTIONS_2024/2025 Tuesday, September 3, 2024 10:30 AM




the log of the ratio of incident light to transmitted light



the ratio of concentration to transmitted light



the ratio of molar absorbance coefficient to lightpath length - Answer✔️✔️-the log of the

ratio of incident light to transmitted light

Beer's Law implies that:



the concentration of a solution is dependent on lightpath length



the distance light travels through a solution is independent of concentration



the molar absorbance coefficient is dependent on the concentration of the absorbing

solute



the absorption of light increases with increasing solute concentration - Answer✔️✔️-the

absorption of light increases with increasing solute concentration

When haemoglobin in oxygenated in the lungs it is most likely to: - Answer✔️✔️-

A 3 mL cuvette was filled with 0.5 mL of solution Y and 2.5 mL of water and its

absorbance at 340 nm was 0.394.

The molar absorbance coefficient of solution Y is 31,000 L/mol/cm. The pathlength is 1

cm.


Page | 2

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