CHEM 219 Organic Bio 219 Final questions with answers A+ graded
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Course
CHEM 219 Organic Bio 219
Institution
CHEM 219 Organic Bio 219
What two things need to be oxidized for fluorescence to occur? - -
aldehyde
-flavin mononucleotide
Luciferase - -enzyme that catalyzes the light emitting reaction
-has alpha and beta subunits
Lux I - -encodes for auto-inducer
Lux R - -encodes for transcriptional activator that binds the auto ...
What two things need to be oxidized for fluorescence to occur? - ✔✔-
aldehyde
-flavin mononucleotide
Luciferase - ✔✔-enzyme that catalyzes the light emitting reaction
-has alpha and beta subunits
Lux I - ✔✔-encodes for auto-inducer
Lux R - ✔✔-encodes for transcriptional activator that binds the auto inducer
-transcribed oppositely all other subunits
Two aspects of gram-negative bacteria - ✔✔-external LPS layer
(lipopolysaccharide)
-single layer of peptidoglycan based cell wall
Operon - ✔✔-genetic regulatory system in which genes coding for
functionally related proteins are clustered along the DNA
Beneficial aspects of bioluminescence - ✔✔-communication
-attracting prey
-hiding from predators
-attracting a mate
, -reliable food source for bacteria (glucose + amino acids)
Two types of organisms that use bioluminescence - ✔✔-pinecone fish
-hawaiian bobtail squid
Quorum Sensing - ✔✔-regulation of gene expression in response to
changes in cell-population density
Auto-induction - ✔✔-bacteria release a small metabolic product, N-Acyl
homoserine lactone, which diffuses across membrane into extracellular
environment
Two Part Purpose of class - ✔✔-create a complete Aliivibrio fischeri
genomic library
-create a restriction map of lux containing plasmid
Lux D - ✔✔-encodes for the acyl-transferase enzyme
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