CMB2001 Questions and Answer | Latest Update 2024/2025
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Course
CMB2001
Institution
CMB2001
Eukaryotic promoters have a basal region which can contain a TATA box,
initiator (Lnr), BRE (TFIIV recognition element) and what other element?
~~> Downstream core promoter (DPE)
In prokaryotes RNA polymerase requires what co-factor?
~~> 70 sigma factor
Forms the holoenzyme
The prom...
After RNA polymerase transcribes and clears the promoter what is
hyperphosphorylated?
✓ ~~> The C-terminal domain
TFIIH has two parts ATPase called XPB which causes promoter melting, CORE
region and what other region?
✓ ~~> CAK - the kinase that phosphorylates the C- terminal domain
TFIID has a trilobular structure and binds the TATA box, what other factors bind
to it?
✓ ~~> TATA binding protein (TBP is the main subunit), TBP associated
factors (TAFs) = interact with elements which bind to the promoter
region
TAF6 = DPE
Promoters with only the basal or core regions will only have low levels of
transcription, enhancer elements can increase the level of transcription.
Master01 | September, 2024/2025 | Latest update
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