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MLS ASCP Exam - MICRO With Questions And Accurate Answers
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MLS ASCP Exam - MICRO With Questions And Accurate AnswersDisinfection, desiccation, filtration, fridge, heat, radiation, and Sterilization - Answer
Types of sterilization/inhibition techniques
denatures proteins
15 lbs ,121*C for 15 mins - Answer Moist Heat Sterilization
kills by oxidation
hot air oven 170*C for 2 hrs - Answer dry heat sterilization
140*C for 3 secs, NOT STERILIZATION - Answer pasturization sterilization
Damages cytoplasmic membrane and denatures protein - Answer Disinfectant: Phenol
Oxidizes (iodine and chlorine) - Answer Disinfectant: Halogens
Denatures proteins and dissolves lipids - Answer Disinfectant: Alcohols
penicillins and cephalosporins
cell wall synthesis - Answer B Lactams
Vancomycin
C Diff/MRSA
cell wall synthesis - Answer Glycoproteins
Gentamycin, tobramycin - Answer Aminoglycosides
-Tetracycline, doxycycline, minocycline
-Clinical use: Borrelia burgdorferi, M. pneumoniae. Drugs' ability to accumulate
intracellularly makes them very effective against Rickettsia and Chlamydia. Also used to
treat acne. - Answer Tetracyclines
,Meningitis (Haemophilus influenzae, Neisseria meningitidis, Streptococcus
pneumoniae) and Rocky Mountain spotted fever (Rickettsia rickettsii). - Answer
Chloramphenicol
erythromycin, clarithromycin, azithromycin - Answer Macrolides
Ciprofloxacin, norfloxacin
P aeruginosa - Answer Quinolines
UTI (Bactrim, sulfisoxazole)
Ulcerative colitis (Sulfasalazine)
folic acid metabolism - Answer Sulfa drugs
quinupristin/dalfopristin
vanco resistant E. Faecium - Answer Streptogramins
linezolid (Zyvox);
Used to treat methicillin resistant Staphylococcus aureus (MRSA) and vancomycin
resistant Enterococcus (VRE) - Answer Oxazolidinones
Aminoglycosides, cephalosporins, quinolones - Answer GNR Drugs
, RNA metabolism - Rifampin
killing harmful microbes without damaging the host - selective toxicity
Kills bacteria without host immune help - Bactericidal action
Inhibits organism growth - Bacteriostatic action
the minimum concentration of a substance necessary to prevent microbial growth -
Minimum inhibitory concentration MIC
minimum concentration of drug needed for killing of bacteria - Minimum bactericidal
concentration MBC
a more resistant group of beta-lactamases that hydrolyze extended spectrum
cephalosporins w/ an *oxyimino side chain*
(abx ex: cefotaxime, ceftriaxone, ceftazidime, aztreonam) - Answer ESBL (extended
spectrum beta-lactamase)
Test used to detect inducible clindamycin resistance in staph and β-streptococci.
Erythromycin disk in placed next to clindamycin disk on std. disk diffusion plate.
Flattening of the clind. zone between the two disks indicates inducible clindamycin
resistance. - Answer D Test
organisms that have become resistant to carbapenems, which are a class of antibiotics
K. Pneumo and E coli - Answer Carbapenem-resistant Enterobacteriaceae (CRE)
Swarming proteus zone around penicillin or ampicillin discus with B-Lactamase neg
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