Exam (elaborations)
(MB) ASCP PRACTICE EXAM QUESTIONS AND ANSWERS
- Course
- Institution
(MB) ASCP PRACTICE EXAM QUESTIONS AND ANSWERS
[Show more]
Content preview
(MB) ASCP PRACTICE EXAMQUESTIONS AND ANSWERS
Which0of0the0following0is0not0a0component0of0a0nucleotide?
Phosphate0group
Anti-codon
Ribose0sugar
Nitrogen0base0-0ans--✔✔Anti-codon
According0to0Chargaff's0rule0of0base0pairing,0adenine0pairs0with:0-0ans--✔✔Thymine
What0genes0would0be0screened0in0a0breast0cancer0panel?0-0ans--
✔✔HER2,0ERBB2,0BRCA1
Next0Generation0Sequencing0uses:0-0ans--✔✔Short0sequence0reads
Purines0and0pyrimidines0differ0from0each0other0in0that:0-0ans--
✔✔Purines0have0two0rings;0pyrimidines0have0one0ring
The0purines0are:
Cytosine0and0uracil
Adenine0and0thymine
Thymine0and0cytosine
Adenine0and0guanine0-0ans--✔✔Adenine0and0guanine
What0is0the0rate0of0mutation0per0round0of0DNA0replication?
10in01,0000base0pairs
10in010,0000base0pairs
10in01,000,0000base0pairs
10in01,000,000,0000base0pairs0-0ans--✔✔10in01,000,000,0000base0pairs
The0rate0of0DNA0migration0through0an0agarose0gel0during0electrophoresis0does0not0depe
nd0on0which0of0the0following0factors?
Net0charge0of0the0molecule
Size0of0the0molecule
Shape0of0the0molecule
Nucleotide0sequence0of0the0molecule0-0ans--✔✔Nucleotide0sequence0of0the0molecule
,What0are0the0phases0in0a0qPCR0Amplification0Plot?
Initiation,0exponential,0plateau
Baseline,0exponential,0plateau
Baseline,0threshold,0exponential,0plateau
Baseline,0initiation,0threshold,0exponential,0plateau0-0ans--
✔✔Initiation,0exponential,0plateau
Find0the0palindrome0in0this0restriction0enzyme0site:05'-CTGCAG-3'?
5'-GAC
3'-GAC
3'-CTG
5'-GTC0-0ans--✔✔3'-GAC
A0patient0with0impaired0judgment,0personality0changes,0signs0of0abnormal0body0moveme
nts0and0depression0comes0to0the0physician's0office0for0a0follow-
up0visit.0The0physician0suspects0a0single-
gene0disorder0may0be0the0cause0of0those0clinical0manifestations.0A0blood0specimen0was0
then0sent0to0your0clinical0laboratory0for0mutation0screening0in0the0Huntington0gene.0Testi
ng0with0standard0PCR0indicates0that0patient0has0Huntington0Disease,0HD.0Which0of0the0f
ollowing0would0be0consistent0with0this0diagnosis?
250CAG0repeats0in0the0Huntington0gene
850CAG0repeats0in0the0Huntington0gene
250CGA0repeats0in0the0Huntington0gene
850CGA0repeats0in0the0Huntington0gene0-0ans--
✔✔850CAG0repeats0in0the0Huntington0gene
Which0two0HPV0types0are0responsible0for0most0cases0of0cervical0cancer?
160and018
310and059
160and058
440and0590-0ans--✔✔160and018
Replication0forks,0known0as0origins0of0DNA0replication,0are0created0by0this0enzyme:
Ligase
Taq0Polymerase
Primase
Helicase0-0ans--✔✔Helicase
Mutation0in0what0gene0is0associated0with0Fragile0X0syndrome?0-0ans--✔✔FMR1
Mantle0cell0lymphoma0(MCL)0is0caused0by0what0translocation?0-0ans--✔✔t(11;14)
This0polymerase0is0involved0in0"initiation0of0DNA0replication0and0has0primase0activity":0-
0ans--✔✔Pol0α
, Its0discovery0shed0light0on0why0there0is0simultaneous,0though0not0continuous,0synthesis0
of0DNA0on0both0leading0and0lagging0strands0of0DNA:
Klenow0fragment0of0DNA0polymerase
Okazaki0fragments
Sanger0fragments
RNA0fragments0-0ans--✔✔Okazaki0fragments
What0gene0is0measured0following0treatment0with0imatinib0(Gleevec)?
FLT3
BCR/Abl
Jak2
MAPK0-0ans--✔✔BCR/Abl
What0is0the0rate0of0mammalian0DNA0replication?
5000nucleotides0per0second
1000nucleotides0per0second
500nucleotides0per0second
100nucleotides0per0second0-0ans--✔✔500nucleotides0per0second
This0polymerase0is0involved0in0"replicates0mitochondrial0DNA":0-0ans--✔✔Pol0γ
A0patient0with0impaired0judgment,0personality0changes,0signs0of0abnormal0body0moveme
nts0and0depression0comes0to0the0physician's0office0for0a0follow-
up0visit.0The0physician0suspects0a0single-
gene0disorder0may0be0the0cause0of0those0clinical0manifestations.0A0blood0specimen0was0
then0sent0to0your0clinical0laboratory0for0mutation0screening0in0the0Huntingtin0gene.0Which
0of0these0methods0would0best0accomplish0this0task?
Methylation-specific0PCR
Standard0PCR
PFGE
RAPD0PCR0-0ans--✔✔Standard0PCR
Which0of0the0following0storage0options0is0optimal0for0storing0isolated0DNA0for0a0period0gr
eater0than0seven0years?
22-25ºC
2-8ºC
-20ºC
-70ºC0-0ans--✔✔-70ºC
Consider0a0hypothetical0mutation0involving0gene0X.0Let's0say0you0amplify0a0specific0exon
,0say0exon011,0of0that0gene0then0you0cut0it0with0restriction0enzyme0W.0In0a0person0withou
t0the0mutation,0cutting0the0gene0with0restriction0enzyme0W0generates0two0fragments0of0si
zes,01000bp0and02500bp.0Suppose0a0C>T0mutation0in0gene0X0deletes0a0restriction0site,0yi
elding0a0fragment0of03500bp.0You0would0expect0a0heterozygous0person0for0gene0X0to0hav
e0these0fragments0on0a0restriction0gel:
+/+0=03500bp;02500bp;01000bp;
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller MASTERGRADE01. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $11.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
62890 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now