Exam (elaborations)
CCBC Microbiology 230-Summer 2011-Questions #2 Study Set
- Course
- Institution
CCBC Microbiology 230-Summer 2011-Questions #2 Study Set ...
[Show more]
Content preview
CCBC Microbiology 230-Summer2011-Questions #2 Study Set
Why do we heat fix the bacteria to the slide before staining? - Answer So the bacteria
will hold the stain better.
A basic dye has the color in the
_____________ ion - Answer Positive
All bacteria have a slight negative charge; a basic dye has the color in the positive ion.
Opposite charges attract. This is the principle behind: - Answer direct staining
Why do gram positive bacteria appear purple after staining? - Answer The alcohol and
acetone dehydrates
the many layers of peptidoglycan
A bacterial that has one flagella is called what? - Answer Monotrichis.
A bacteria that has flagella all around its outside coat (surrounded by flagella) is called
what? - Answer Peritrichas.
A bacteria that has a flagella that is coming out both ends is called what? - Answer
Lophotricus.
A bacteria that has a single flagella at both poles is called what? - Answer
Amphitrichous.
What are the steps for staining a capsul? - Answer 1) put five loops of bacteria on a slide
from a broth mixture
2) Let mixture dry on slide.
3) add two drops of crystal violet, spread on slide.
4) let slide dry for 1-2 min.
5) flood slide with copper sulfate let sit for 20 seconds.
6) Shake off excess copper sulfate.
7) Blot slide dry.
What are the characteristics of a gram positive cell wall? - Answer 1) Thick dense wall
, 2) contains high levels of peptidogylcan.
3) Wall is composed of teichoic acids
What are the characteristics of a gram negative cell wall? - Answer 1) The cell wall only
contains 2-3 layers of peptidogylcan.
2) Phospholipids are found mainly in the inner layer of the outer membrane
What are the steps to gram stain a bacteria? - Answer 1) Stain bacteria with crytal violet
2) Then treat bacteria with gram's iodine solution.
3) Add grams decolorizer
4) Add safranin.
What type of charge do bacteria have? - Answer Bacteria have a slightly negative
charge.
What are the basic dyes? - Answer the basic dyes are methylene blue, crystal violet and
safrain.
What is a direct stain? - Answer The bacteria will hold the stain inside of the cell.
What is an indirect stain? - Answer The bacteria will not let the stain come into its cell,
so the stain colors around the bacteria.
What are the steps to directly stain bacteria? - Answer 1) Put one drop of water on a
slide
2) aseptically remove some bacteria and mix it into the water.
3) Spread the mixture over the slide.
4) Let slide dry.
5) Pass slide through flame 12 times to heat fix bacteria.
6) Cover slide with safrain and let sit for 1 minute.
7) Wash slide.
8) Blot slide dry.
What are the steps to indirectly stain bacteria? - Answer 1) Put one drop of nigrosin on
slide.
2) Aseptically add a small amount of bacteria and mix with dye.
3) Use another slide to spread bacteria on slide.
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Zayla. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $9.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
77333 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now