what is the workflow for histology (7)️️1) specimen collection
2) grossing
3) fixation
4) processing
5) embedding
6) microtomy
7) routine staining
how are specimens received?️️they are removed from the body and sent to receiving area
of the lab
-in specimen container
-if the speci...
Histology - OSMTwith comprehensive questions and answers
what is the workflow for histology (7)✔️✔️1) specimen collection
2) grossing
3) fixation
4) processing
5) embedding
6) microtomy
7) routine staining
how are specimens received?✔️✔️they are removed from the body and sent to receiving area
of the lab
-in specimen container
-if the specimen is sent fresh and no fixed they are in saline or saline gauze
characteristics of a specimen container (5)✔️✔️-leak proof, screw cap lid
-fixative or saline inside
-wide mouth
-WHMIS label
-patient id label
what is on a specimen container label have on it (7)✔️✔️-pt name
-DOB
-hospital #
-collection date
-specimen type
-ordering doctor
,-whmis or ghs label
what should the req match✔️✔️the specimen container and specimen type
do all the forms for a specimen type being received in the lab have to match ?✔️✔️yes all
forms, container and req must match
where do tissue reception come from✔️✔️comes from operating room ,ER, doctor office and
clinics
what is each specimen given thats unique when its being recieved into the lab✔️✔️given an
unique assesion #
each specimen is checked to make sure it all confirms
what would be an example of an unique assesion number for a specimen coming from
autopsy✔️✔️A2018-3
what are specimens called in order to remove it , it has to be done by
surgery✔️✔️irretrievable specimen
what is grossing✔️✔️looking at the sample in greater detail
-examining and describing
what are the 7 key components in grossing✔️✔️1) # of pieces
2) size
3) weight
4) colour
, 5) texture/consistency
6) cut surface
7) what is being submitted for work up
what are block sections✔️✔️sections that are cut from the tissue that are being examined for
interest
what is the thickness of an ideal block section✔️✔️3-4 mm thick
where are block sections placed into✔️✔️cassettes
what are some specimens (objects) that are not processed✔️✔️bullets, implants, impaled
objects
what is fixation✔️✔️to examine tissue thoroughly to harden and preserve
what is the aim of fixation✔️✔️to preserve the tissue in a lifelike manner
what the key of fixation✔️✔️stabilize proteins
what is the goal of fixation✔️✔️to prevent putrefaction which is the destruction of cells by
bacterial pathogens or normal flora
what are the effects of fixation (4)✔️✔️1) hardens tissue
2) makes tissue easier to work with
3) protects tissue from hard chemicals
4) enhances staining by making the tissue more receptive to dyes
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