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MCB 301 Final Exam Questions and Answers | 100% Pass

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MCB 301 Final Exam Questions and Answers | 100% Pass Which of the following statements about antibiotics is TRUE? - Answer️️ -Incorrect Some antibiotics work by binding the 40S ribosomal subunit. Incorrect Most of the earth's microorganisms have been tested for antibiotic production. Incor...

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  • September 18, 2024
  • 17
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • MCB 301
  • MCB 301
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EmillyCharlotte
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FIRST PUBLISHED: SEPTEMBER 2024

MCB 301 Final Exam Questions and
Answers | 100% Pass

Which of the following statements about antibiotics is TRUE? - Answer✔️✔️-Incorrect

Some antibiotics work by binding the 40S ribosomal subunit.

Incorrect Most of the earth's microorganisms have been tested for antibiotic production.

Incorrect Antibiotics are used to treat fungal infections.

Incorrect Antibiotics are used to disinfect bench tops.

Correct: Some antibiotics are not produced by microorganisms.

In the MCB301 exercise, soil isolates will be tested to determine if they have

antimicrobial activities against which Gram-negative bacteria? - Answer✔️✔️-Incorrect

Salmonella typhimurium

Incorrect Streptomyces sp.

Correct: Pseudomonas aeruginosa

Incorrect Mycobacterium smegmatis

Incorrect Proteus vulgaris

Which of the following ingredients is not present in the enrichment medium that will be

used in the MCB301 lab to isolate antibiotic producing bacteria? - Answer✔️✔️-Incorrect

ZnSO4·7H2O

Correct: yeast extract

Incorrect glycerol

,TITLE: EMILLYCHARLOTTE 2024/2025 ACADEMIC PERIOD
OWNER: EMILLYCHARLOTTE
COPYRIGHT STATEMENT: ©2024 EMILLYCHARLOTTE. ALL RIGHTS RESERVED
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Incorrect arginine monohydrochloride

Incorrect potassium phosphate

Which gene will be amplified and sequenced to help identify the bacterium isolated in

the MCB301 lab? - Answer✔️✔️-Incorrect pepM gene

Incorrect ribosomal protein gene

Correct: a gene that encodes an RNA found in ribosomes

Incorrect tRNA gene

Incorrect beta-lactam gene

Based on the additional readings assigned for this exercise, initial cultures of the fungus

that produced penicillin were grown in large amounts, but the activity of penicillin was

low. Why? (2 pts) - Answer✔️✔️-The culture was contaminated with E. coli (1 pt) that

produced an enzyme that degraded penicillin (1 pt).

Based on their mechanisms of action, how are clindamycin and lincomycin similar to

amikacin and gentamicin, and how are they different? - Answer✔️✔️-They are similar in

that they all inhibit protein synthesis. (1 pt)



They are different in that clindamycin and lincomycin bind to the large 50S ribosomal

subunit of bacteria, and amikacin and gentamicin bind to the small 30S ribosomal

subunit. (1 pt)

, TITLE: EMILLYCHARLOTTE 2024/2025 ACADEMIC PERIOD
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COPYRIGHT STATEMENT: ©2024 EMILLYCHARLOTTE. ALL RIGHTS RESERVED
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Arsenic can affect the growth of bacteria, but it is not considered an antibiotic. Why? -

Answer✔️✔️-Arsenic is not selective against just bacteria (like antibiotics), but it is also

toxic to the eukaryotic host. (2 pts)

Why does Mycobacterium smegmatis not Gram stain well? What is the name of the

type of staining normally used to stain this bacterium? Note: To fully answer this

question you will have to check the Brock textbook or other reliable source. -

Answer✔️✔️-The surface of Mycobacterium contains lots of mycolic acids bound to the

peptidoglycan. (1 pt)



To properly stain Mycobacterium it should be acid-fast stained. (1 pt)

One of the assigned readings described genome mining and its use to discover

potentially new phosphonic acid products. The genomes of Actinomycetes were

searched for which gene and why? - Answer✔️✔️-The pepM gene (gene that encodes

phosphoenolpyruvate mutase) was used (1 pt) because known phosphonate

biosynthetic pathways often involve this gene product (1 pt).

Which of the following does not contain 16S rRNA: viruses, archaea, bacteria, eukarya?

Also, explain why? - Answer✔️✔️-Viruses do not have ribosomes (1 pt).



Eukarya have 18S rRNA (1 pt).

pRL27 contains all of the following EXCEPT

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