Straighterline Microbiology Lab BIO250L Lab 4 Selective Media & Agar Worksheet
(New Version Updated)
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Lab Report Format Expectations
Utilize college level grammar and professional formatting when completing this worksheet.
Submissions without proper formatting, all required photos or sufficient responses will be rejected.
Pre-lab Questions
1. In this lab, you will conduct three different experiments that each cover the topic of selective
media and agar. How are these experiments different from one another? Explain how the
different media in each experiment are utilized in the context of selective vs differential media.
The precise goals and kinds of microorganisms each experiment aims to isolate vary. The
selection of media is based on their capacity to promote the development of particular bacteria
(selective growth) or facilitate the differentiation of bacterial traits (differential expansion). Each
experiment's media selection is based on the specific characteristics of the target bacterium.
They have a clear emphasis on identifying and isolating microorganisms with particular
characteristics. Starch agar is used in the bioprospecting experiment to promote the
development of bacteria that specifically break down starch. In Experiment 2, Gram-positive
bacteria are isolated and distinguished according to mannitol fermentation and hemolysis
patterns using Mannitol Salt Agar and Columbia CNA Agar. In Experiment 3, Gram-negative
bacteria are isolated and distinguished using MacConkey Agar and Eosin Methylene Blue Agar as
selective media. The classification process is based on lactose fermentation and colony
appearance. To further our understanding of the diversity and features of microbes, every
experiment uses a combination of selective and differential media specific to the target
microorganisms.
2. What is the difference between chemically defined and chemically complex media? Give an
example (a clinical or environmental research application) of when you would use each type of
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, Lab 4 Selective Media & Agar BIO250L
medium to culture different microorganisms.
Chemically defined media are distinguished from chemically complicated media by their
chemical composition. Chemically defined media, like glucose broth, have well-established
designs. Conversely, chemically complicated media, like wholesome broth, might vary from
batch to batch and have unknown ingredients.
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