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MCB 301 Exam III Questions and Answers | 100% Pass

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MCB 301 Exam III Questions and Answers | 100% Pass In the enrichment portion of the Experiment, how many nutrient agar plates will be inoculated from your vegetation sample? - Answer️️ -Incorrect 5 Incorrect 3 Incorrect 4 Correct: 2 In the isolation portion of the Experiment, wet mounts ...

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  • September 18, 2024
  • 17
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • MCB 301
  • MCB 301
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EmillyCharlotte
TITLE: EMILLYCHARLOTTE 2024/2025 ACADEMIC PERIOD
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COPYRIGHT STATEMENT: ©2024 EMILLYCHARLOTTE. ALL RIGHTS RESERVED
FIRST PUBLISHED: SEPTEMBER 2024

MCB 301 Exam III Questions and
Answers | 100% Pass


In the enrichment portion of the Experiment, how many nutrient agar plates will be

inoculated from your vegetation sample? - Answer✔️✔️-Incorrect 5


Incorrect 3

Incorrect 4

Correct: 2

In the isolation portion of the Experiment, wet mounts will be made from potential spore-

forming cells that grew on the plates that were - Answer✔️✔️-Incorrect incubated

anaerobically.

Incorrect incubated aerobically and anaerobically.

Correct: incubated aerobically.

In the post-enrichment portion of the Experiment, your spore-forming isolate will be

inoculated into how many tubes and flasks of medium? - Answer✔️✔️-Incorrect 4

Correct: 5

Incorrect 2

Incorrect 3

Incorrect 1

,TITLE: EMILLYCHARLOTTE 2024/2025 ACADEMIC PERIOD
OWNER: EMILLYCHARLOTTE
COPYRIGHT STATEMENT: ©2024 EMILLYCHARLOTTE. ALL RIGHTS RESERVED
FIRST PUBLISHED: SEPTEMBER 2024

What should be the Gram reaction of the spore-formers we isolate? - Answer✔️✔️-

Correct: Gram-positive rods

Incorrect Gram-positive cocci

Incorrect Gram-negative rods

Incorrect Gram-negative cocci

What are the two most common genera of bacteria that can make endospores, and

describe some common characteristics between the two genera (unrelated to spore

formation)? (2 pts) - Answer✔️✔️-Bacillus and Clostridium (1 pt)



Both are Gram-positive bacteria, rod-shaped and most are motile (1 pt)

Is sporulation a form of reproduction? Why or why not? - Answer✔️✔️-No, because

sporulation involves a single vegetative cell forming into a single spore (1 pt), instead of

a single cell forming two viable cells as in reproduction (1 pt).

During sporulation, an inner forespore membrane and an outer forespore membrane

are formed (although one of them is degraded later). Where did each membrane come

from, and structurally how do the two membranes in the forespore differ from each

other? (2 pts - Answer✔️✔️-The inner forespore membrane came from the original

daughter cell membrane, and the outer forespore membrane came from the mother cell

membrane. (1 pt)

, TITLE: EMILLYCHARLOTTE 2024/2025 ACADEMIC PERIOD
OWNER: EMILLYCHARLOTTE
COPYRIGHT STATEMENT: ©2024 EMILLYCHARLOTTE. ALL RIGHTS RESERVED
FIRST PUBLISHED: SEPTEMBER 2024
The outer forespore membrane is actually inside out compared to the inner forespore

membrane. (1 pt)




Suring sporulation, the membrane of the mother cell grows around the membrane of the

daughter cell. As a result, the daughter cell is engulfed inside the mother cell and is

surrounded by two membranes : the inner foreshore membrane, which arises from it's

own cytoplasmic membrane, and the outer foreshore membrane which arises from the

inside-out cytoplasmic membrane of the mother cell

In the sporeformer post-enrichment exercise, PYE medium was supplemented with

glucose. How many milliliters (mL) of a 25% glucose stock solution must be added to 20

mL of PYE medium to make 0.4% glucose? (2 pts) - Answer✔️✔️-0.32 ml (2 pts)



Note: (25%)(x mL) = (0.4%)(20 mL) = 0.32 mL




C1V1=C2V2, (25% glu) X = (0.4% final glu) (20 mL PYE), X = 0.32 mL

Why are cysteine residues important in the spore coat protein? (2 pts) - Answer✔️✔️-

Cysteine residues contain sulfhydryl groups so two residues can covalently link to each

other by forming a disulfide bond. This allows the coat proteins to interact more strongly

with one another to strengthen the spore coat. Also, intramolecular disulfide bonds

might form to strengthen the individual coat protein. (2 its)

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