MCB 301 Exam study Guide with
complete solutions
selectable marker - Answer️️ -confers a detectable growth trait on organisms that
possess them; ofter confers a resistance to an antibiotic that the organism previously
didn't have--- present on all cloning plasmids
origin of replication - A...
DNA fingerprinting steps - Answer✔️✔️-1) digest dna smaller via restriction enzymes
2) separate fragments by size
3) visualize bands
4) unique pattern of bands identifies donor of dna
nucleic acid hybridization - Answer✔️✔️-can dna dna from x hybridize with dna from y? if
so then x may be similar to y
PCR - Answer✔️✔️-Primers used in PCR must hybridize to complementary sequences to
amplify the region of DNA
in our lab we identify bacteria by..? - Answer✔️✔️-- Morphological Characteristics
- Differential Staining (Gram stain)
- Biochemical Tests (Selective and differential media & Enzymatic tests)
Drawbacks of a dichotomous key - Answer✔️✔️-Time consuming; genomic mutation or
plasmid may change characteristics and give false results; Alternative: Best fit method
using several tests at once Example: Enterotube
southern blot - Answer✔️✔️-1) restriction enzyme digestions
2) agarose gel electrophoresis
3) southern blot transfer
4) hybridization with probes
Agarose - Answer✔️✔️-long polymer of galactose units
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