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MB (ASCP) EXAM ATCUAL EXAM 300 QUESTIONS AND CORRECT DETAILED ANSWERS (100% VERIFIED ANSWERS)

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MB (ASCP) EXAM ATCUAL EXAM 300 QUESTIONS AND CORRECT DETAILED ANSWERS (100% VERIFIED ANSWERS) Predominant form of DNA - Answer️️ -Right-handed B DNA and histone interaction is due to what type of bond? - Answer️️ -Ionic What stains to the minor groove of DNA duplex? - Answer️️ -S...

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  • September 18, 2024
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MB (ASCP) EXAM 2023-2024 ATCUAL
EXAM 300 QUESTIONS AND CORRECT
DETAILED ANSWERS (100% VERIFIED
ANSWERS)


Predominant form of DNA - Answer✔️✔️-Right-handed B


DNA and histone interaction is due to what type of bond? - Answer✔️✔️-Ionic

What stains to the minor groove of DNA duplex? - Answer✔️✔️-SYBR Green

What is the most abundant type of RNA? - Answer✔️✔️-Ribosomal RNA (rRNA)

What does RNA act as in a retrovirus? - Answer✔️✔️-Genome and mRNA

What increases half-life of mRNA? - Answer✔️✔️-5' Methyl Cap

What kind of DNA is most likely to be transcriptionally active? - Answer✔️✔️-Euchromatin

What is the most common type of eukaryotic DNA? - Answer✔️✔️-Satellite DNA

What specimen has the most DNA according to molecular weight? - Answer✔️✔️-White

Blood Cells

What amino acids compose histones? - Answer✔️✔️-Lysine and Arginine

Amino acid that all proteins start with: - Answer✔️✔️-Methionine

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OWNER: EMILLYCHARLOTTE
COPYRIGHT STATEMENT: ©2024 EMILLYCHARLOTTE. ALL RIGHTS RESERVED
FIRST PUBLISHED: SEPTEMBER 2024

What protects the DNA strand during replication? - Answer✔️✔️-Single-stranded binding

protein

Leading Strand - Answer✔️✔️-3' to 5' template

Lagging Strand - Answer✔️✔️-5' to 3' template

What DNA strand is being copied in transcription? - Answer✔️✔️-Antisense Strand

First step after newly formed mRNA is made? - Answer✔️✔️-Addition of poly(A) tail

Every time the chromosome is copied it would become shorter if not for what enzyme? -

Answer✔️✔️-Telomerase

What polymerase has repair activity? - Answer✔️✔️-Beta/Epsilon

What polymerase acts on leading strand? - Answer✔️✔️-Delta

What polymerase acts on lagging strand? - Answer✔️✔️-Alpha

Which polymerase is used for mitochondrial replication and repair? - Answer✔️✔️-

Gamma

What polymerase binds the 5' end to 3' end of dsDNA? - Answer✔️✔️-Topoisomerase

Which RNA polymerase is mainly for transcription? - Answer✔️✔️-RNA Pol II

Which polymerase is involved with mRNA synthesis? - Answer✔️✔️-RNA Pol II

What inactivates RNAses? - Answer✔️✔️-Diethyl pyrocarbonate (DEPC) or bake

glassware for 4 to 6 hours at 400C

What type of restriction enzyme recognizes palindromic sites? - Answer✔️✔️-Restriction

Enzyme Type II

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What type of restriction enzyme lacks methylation activity? - Answer✔️✔️-Restriction

Enzyme Type II

What is necessary to make cDNA? - Answer✔️✔️-Reverse transcriptase

Number of SNPs in a human genome? - Answer✔️✔️-11 billion (10^7)

How many genes (base pairs) are in a human haploid genome? - Answer✔️✔️-3 billion

base pairs

The polymerization of acrylamide requires: - Answer✔️✔️-Catalyst (APS or TEMED) or

Light activation

How does agarose gel pore size relate to concentration? - Answer✔️✔️-Inversely

proportional

What is denatured during a southern blot? - Answer✔️✔️-Hydrogen bonds (inter-strand

bonds)

Melting temperature formula - Answer✔️✔️-Tm = (4C x GC Pairs) + (2C x AT pairs)

Which is likely to interfere with fluorescent probe? - Answer✔️✔️-5' G

Proper GC content of primer - Answer✔️✔️-35-65%

Inhibitors of PCR - Answer✔️✔️-Hemoglobin, Heparin, Xylene, Phenol, Cyanol, and

Bromophenol Blue

Order to add to a PCR reaction: - Answer✔️✔️-Master mix > patient sample > positive

control > negative control

Pre Area - Answer✔️✔️-Positive Pressure

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Post Area - Answer✔️✔️-Negative Pressure

Which PCR requires two steps for specificity? - Answer✔️✔️-Nested PCR

Which microorganism is detected by branched DNA amplification? - Answer✔️✔️-DNA or

RNA

Which technique uses RNA, antibodies, and DNA? - Answer✔️✔️-Hybrid Capture Assay

Use of bisulfite treatment of DNA to determine its pattern of methylation - Answer✔️✔️-

Bisulfite DNA

What tests are isothermal? - Answer✔️✔️-NASBA, TMA, & SDA

Which leukemia is associated with FLT3 gene? - Answer✔️✔️-AML

T-cell gene rearrangement is seen in what malignancy? - Answer✔️✔️-Sezary Syndrome

Which translocation causes Acute Premyelocytic Leukemia? - Answer✔️✔️-t 15;17

Ewing Sarcoma - Answer✔️✔️-t 11;22

Synovial Sarcoma - Answer✔️✔️-t X;18

Follicular Lymphoma - Answer✔️✔️-t 14;18

What genes would be screened in a colorectal panel? - Answer✔️✔️-MSH2 & MLH1

What gene mutation causes a high risk of breast or ovarian cancer? - Answer✔️✔️-

BRCA1 and BRCA2

What does MSI test for? - Answer✔️✔️-Mutations in mismatch repair genes (MMR)

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