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Biochemistry ACS Review Questions With 100% Complete Solutions

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  • Biochemistry
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  • Biochemistry

Biochemistry ACS Review Questions With 100% Complete Solutions A codon is present in _______. An anticodon is found in _______. The 5' - AUC - 3' codon, will base pair with the 5' - _______ - 3' anticodon. A. mRNA, tRNA, UAG B. mRNA, tRNA, GAT C. DNA, mRNA, GAU D. mRNA, tRNA, GAU E. DNA...

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  • September 19, 2024
  • 28
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • Biochemistry
  • Biochemistry
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A codon is present in _______. An anticodon is found in _______. The 5' - AUC - 3'
codon, will base pair with the 5' - _______ - 3' anticodon.

A. mRNA, tRNA, UAG
B. mRNA, tRNA, GAT
C. DNA, mRNA, GAU
D. mRNA, tRNA, GAU
E. DNA, mRNA, CUA - Answer-D. mRNA, tRNA, GAU

Anticodon is reversed because it is running 5'-3

/.A researcher wants to determine if cancer cells express protein X. Which technique
would be the most specific way of determining this?

A. Northern blotting
B. Southern blotting
C. Western blotting
D. UV/V spectroscopy
E. Bradford protein assay - Answer-C. Western blotting

/.All of the compounds represent different metabolites of pyruvate except:

A. Acetyl-CoA
B. Alanine
C. Glutamate
D. Lactate
E. Oxaloacetate - Answer-C. Glutamate

/.All of these are required for fatty acid synthesis except

A. acetyl-CoA
B. acyl carrier proteins
C. ATP
D. malonyl-CoA
E. NADH - Answer-E. NADH

/.All of these enzymes in the TCA cycle require NAD+ except

A. Malate dehydrogenase
B. Succinate dehydrogenase

,C. Isocitrate dehydrogenase
D. alpha-ketoglutarate dehydrogenase
E. All of the above require NAD+ - Answer-B. Succinate dehydrogenase

Generates ubiquitol

/.As glucose levels in the blood begin to drop, glucagon is released from the alpha cells
of the pancreas. Release of this polypeptide hormone helps increase the availability of
ATP-producing compound by

A. inhibiting acetyl-Coa carboxylase causing an increased in beta-oxidation
B. stimulating glycogen phophorylase causing the release of glucose from glycogen
C. stimulating hormone-sensitive triacylglycerol lipase in adipose cells causing the
release of fatty acids
D. all of the above
E. none of the above - Answer-D. all of the above

/.Cheese is produced by adding bacteria and enzymes to milk. The bacteria and
enzymes create lactate and H+ that causes the pH of milk to decrease, and the proteins
to precipitate to form soft curds. This is an example of using a protein's _______ to
purify it.

A. density
B. hydrophobicity
C. instability
D. isoelectric point
E. relative molar mass - Answer-D. isoelectric point

/.During heavy exercise, muscle tissue can undergo anaerobic metabolism to produce
lactate. What is the fate of this lactate?

A. It is converted back to pyruvate for gluconeogenesis in the muscle
B. It is transported to the liver for gluconeogenesis to produce glucose
C. It is converted to acetyl-CoA for the production of fatty acids to produce ATP
D. It is used as a carbon source for hte synthesis of amino acids for protein production
E. It is stored in the muscle tissue until oxygen rise and it can be used by the TCA cycle
- Answer-B. It is transported to the liver for gluconeogenesis to produce glucose

/.During starvation or diabetes mellitus the brain derives most of its energy from

A. fatty acids
B. glucose
C. ketone bodies
D. glycogen
E. fructose - Answer-C. ketone bodies

, /.Familial hypercholesterolemia is a disease caused by an error in metabolism that
leads to elevated levels of cholesterol in the blood. It is considered to be caused by

A. diets rich in cholesterol
B. plaque formed in the arteries
C. elevated cholesterol biosynthesis in liver tissues
D. defective low density lipoprotein (LDL) synthesis
E. defective low density lipoprotein (LDL) receptors - Answer-E. defective low density
lipoprotein (LDL) receptors

/.Histones are proteins that make up nucleosomes. They bind to the backbone of DNA
in the chromosomes. A majority of the residues in these proteins must be __________
for this to occur.

A. Hydrophobic
B. Negatively charged
C. Hydrophilic and neutral
D. Positively charged
E. Cysteines - Answer-D. Positively charged

/.How does oxygen bind to hemoglobin?

A. Cooperatively
B. Allosterically - Answer-A. Cooperatively

/.If bacteria is moved from a warm environment to a colder environment, the bacteria
compensate by

A. synthesizing more unsaturated fatty acids for its membrane
B. synthesizing more saturated fatty acids for its membrane
C. increasing its metabolic rate to generate more heat
D. altering the glycerophospholipid:sphingolipid ratio
E. synthesizing more cholesterol for its membrane - Answer-A. synthesizing more
unsaturated fatty acids for its membrane

/.In an SDS-PAGE gel, which proteins migrate the shortest distance?

A. Smaller/lighter proteins
B. Larger/heavier proteins - Answer-B. Larger/heavier proteins

Smaller proteins travel further and more quickly

/.In the inner mitochondrial membrane, electrons are acquired from reduced coenzymes
_______ and _______ and transported through several membrane complexes to
_______ resulting in the production of _______.

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