Exam (elaborations)
CMB1003 Exam Questions And Accurate Solutions
- Course
- Institution
CMB1003 Exam Questions And Accurate Solutions...
[Show more]
Content preview
CMB1003 Exam Questions And Accurate SolutionsWhy study bacteria - Answer Aids understanding of our cells
Microbes everywhere in large amounts
Utilised for biotechnology
Bacteria - Answer No nucleus
Special cell wall
Plasmids
Adapted to produce energy in lots of different ways
Archea - Answer Kind of like eukaryotoes but prokaryotes
Microbes in food - Answer yoghurt
cheese
probiotics
Pickled stuff
Microbes in biotechnology - Answer Insulin
Monoclonal antibodies
vitamins and amino acid production
industrial enzymes and synthetic biology
Positive impact of human microbiome - Answer Baby microbiome breaks down milk
sugar baby can not
,Gram staining - Answer Crystal violet on cell
wash
iodine
wash
alcohol
wash
counterstain (Safranin)
Electron microscope - Answer Sample in vacuum
Electron scatter
create a digital image
When looking at a bacteria it shows the cytoplasm, septum, cell wall and DNA
Issues with electron microscope - Answer Specimen is fixed and dehydrated
Cryoelectronic microscope - Answer Sample flash frozen while alive so appears living
and can see 3D image
Large and expensive
Fluorescent Microscope - Answer Digital detection with good resolution
See specific things depending on dye used
Immunofluorescence - Answer Detect native and wild type proteins
Specific antibody lights up with joins to specific antigen
Green Fluorescent protein - Answer Dye from fluorescent jelly fish DNA incorporated
into gene f interest in bacteria can see time lapse of the gene
,Cell wall bacteria - Answer Different to Eukaryotes so is a very good target for
antibiotics
Essential for growth and maintain osmotic pressure of bacteria so if impeached water
can move in and cause bacteria to lyse
Gram positive - Answer Simple structure
Cytoplasm
Plasma membrane
peptidoglycan wall
Purple after gram staining
Gram Negative - Answer Complex structure
Cytoplasm
Plasma membrane
Peptidoglycan
Outer membrane
Lipopolysaccharide chain
Pink after gram staining
Outside of bacteria wall - Answer S-layer - crystalline lattice of protein acts a seive
Capsules - Answer Polysaccharide attached to cell wall. Act as a biofilm to make
bacteria immunogenic
Pili and fimbriae - Answer Adhere to human cell
Helps with gene transfer between cells
Assist movement
Flagella - rotation C-ring and then helical structure
, Endospores - Answer Model for cellular development
Incredibly resistant and can remain dormant for years
Biofilms - Answer Held together by matrix and promote adhesion and cohesion
Bacteria Genes - Answer DNA condensed in nucleotide and is very small
Replicate DNA very rapidly
Organised into operons
Housekeeping genes - Answer gene in all bacteria
Pan-genomone - Answer Genes that are from other cells - tell you if resistant to
antibiotics
Operons you should know - Answer Lac operon
Trp Operon
oriC
Lac operon - Answer Lac Y = Permease
Lac Z = Enzyme to turn lactose into glucose and galactose
Lac I = control
Trp Operon - Answer 5 genes in genome for when not enough tryptophan
oriC - Answer Chromosome replication origin
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Easton. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $11.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
85073 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now