Exam (elaborations)
UF MCB3020 EXAM 1 ASGHARI QUESTIONS AND ANSWERS
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UF MCB3020 EXAM 1 ASGHARI QUESTIONS AND ANSWERS
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Name: Score:260 Multiple choice questions
Definition 1 of 260
Solid at room temperature (saturated)
Fat (triglyceride)
Polysaccharide
Disaccharide
Glucose
Definition 2 of 260
Stepwise dilution of the culture with water
Serial Dilution
Selective Medium
Fluid-mosaic Model
Filtration
Definition 3 of 260
Adds water molecule, breaking bonds, catabolic pathway
Hydrophilic
Oxygen
Osmosis
Hydrolysis
,Definition 4 of 260
-High boiling point (100 C) Hydrogen bonding raises the boiling point
-Temperature Buffer
-Excellent Solvent (because of polar nature). Water dissolves hydrophilic molecules, but no
good interactions with hydrophobic.
3 Cardinal Growth Temperatures
Cells are mostly made of:
Viruses (Acellular & Non-living)
Unique Properties of Water
Definition 5 of 260
Cell wall made up of peptidoglycan (or murein)
Only bacteria have endospores
Domain: Bacteria
Fluid-Mosaic Model
ABC Transporters
Obligate aerobes
Definition 6 of 260
How large the image is compared to the actual specimen
Magnification
Distortion
Resolution
Elongation
,Definition 7 of 260
Rod-shaped
Vibrio
Bacillus
Coccus
Spirillum
Definition 8 of 260
-Maintains cell shape
-Protects against osmotic stress
Types of light microscopy:
Types of Differential Stains
Infectious diseases
Roles of Cell Wall
Definition 9 of 260
Application of two or more dyes
Differential Staines
Gram Negative
Selective Medium
Gram Negative Coccus
Definition 10 of 260
Combination of two monosaccharides linked together
Microbes
Disaccharide
Polysaccharide
Proteins
, Definition 11 of 260
10^-3, one-thousandth of a meter
Micrometer (um)
Millimeter (mm)
Centimeter (cm)
Nanometer(nm)
Definition 12 of 260
1)Apply the primary stain--Crystal Violet
-Turns all the cells purple
2)Apply a mordant--Iodine
-Combines with the stain
3)Apply decolorizer--Alcohol
-Removes dye from Gram-Negative but not Gram Positive, turning:
-Gram Positive: Purple
-Gram Negative: Transparent
4) Apply a counterstain--Safranin
-Turns:
-Gram Positive: blue-purple
-Gram Negative: Reddish/pink
Robert Koch (Koch's Postulate)
Surface Layer (S-Layer)
Transmembrane Proteins
Steps involved in a Gram Stain
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