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AS Biology (7401/1) Paper 1 Specimen 2024/25-Qs & As
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AS Biology (7401/1) Paper 1 Specimen 2024/25-Qs & As
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AS Biology (7401/1) Paper 1 Specimen 2024/25-Qs & As01 . 1) A technician investigated the effect of temperature on the rate of an enzyme-controlled
reaction. At each temperature, he started the reaction using the same volume of
substrate solution and the same volume of enzyme solution.
Figure 2 shows his results.
Give one other factor the technician would have controlled.
[1 mark] Correct Ans-Concentration of substrate solution / of enzyme
solution / pH;
02 . 2) Calculate the rate of reaction at 25 C.
[2 marks] Correct Ans-1. 2.5/0.04;
2. g dm-3 minute-1 / g dm-3 s-1
;
,AS Biology (7401/1) Paper 1 Specimen 2024/25-Qs & As
02 . 3) Describe and explain the differences between the two curves.
[5 marks] Correct Ans-1. Initial rate of reaction faster at 37 °C;
2. Because more kinetic energy;
3. So more E-S collisions/more E-S complexes
formed;
4. Graph reaches plateau at 37 °C;
5. Because all substrate used up;
03 . 1) Figure 3 summarises the process of meiosis. The circles represent cells and the
structures within each cell represent chromosomes.
01 . 1) Describe how you could use cell fractionation to isolate chloroplasts from leaf tissue. [3
marks] Correct Ans-1. How to break open cells and remove debris;
2. Solution is cold/isotonic/buffered;
3. Second pellet is chloroplast;
, AS Biology (7401/1) Paper 1 Specimen 2024/25-Qs & As
01 . 2) (Figure 1 shows a photograph of a chloroplast taken with an electron microscope.
Figure 1)
Name the parts of the chloroplast labelled A and B.
[2 marks] Correct Ans-1. A stroma;
2. B granum;
01 . 3) Calculate the length of the chloroplast shown in Figure 1.
[1 mark] Correct Ans-(length of chloroplast/length of bar)um
01 . 4) Name two structures in a eukaryotic cell that cannot be identified using an optical
microscope.
[1 mark] Correct Ans-Mitochondrion/ribosome/endoplasmic
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