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BIOL 2320 Lab Exam questions and correct answers
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BIOL 2320 Lab Exam questions and correct answers
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BIOL 2320 Lab ExamTE/SDS solution - correct answer ✔✔SDS in the solution aids in breaking down the yeast cell
Digestion solution - correct answer ✔✔used to re-suspend the cells and provide appropriate conditions
for the activity of proteinase k
Proteinase K solution - correct answer ✔✔digests the proteins present
RNase A solution - correct answer ✔✔removes RNA
Lysis solution - correct answer ✔✔contains components that will ensure complete lysis of the cells. Also
contains chaotropic salts and helps maintain the right pH for the binding of the DNA to the silica
membrane of the column
50% ethanol - correct answer ✔✔facilitates binding of the DNA on to the silica membrane when
prepared lysate is added to the column
Wash buffers - correct answer ✔✔remove any impurities from the DNA bound to the membrane in the
column
Elution buffer - correct answer ✔✔cause the purified genomic DNA to come off the membrane in the
column and flow into the labelled micro-centrifuge tube.
Chelex resin - correct answer ✔✔Prevents DNA degradation by removing contaminants leaving the DNA
in solution
Primers - correct answer ✔✔short sequences (10 to 20 bps in length) that act as the starting point for
DNA synthesis.
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