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microbiology semmelweis exam questions and answers
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microbiology semmelweis exam questions and answers
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microbiology semmelweis examquestions and answers
What does sterilisation mean? - Correct Answers -Killing procedure of any kind of
germs
What does disinfection mean? - Correct Answers -Procedure where the number of the
germs are reduced to a safety level.
Which parameters can influence the effectivity of the sterilisation? - Correct Answers -
The number of the germs, the resistance of the germs, the concentration of the
disinfectants, the presence of the organic materials, the initial time, the presence of the
biofilm.
Parameters of the hot-air sterilisation cupboard protocol? - Correct Answers -180°C; 1
hour, 160°C; 2 hours, 140°C; 3 hours.
Parameters of the autoclaving? - Correct Answers -+ 1 atm overpressure, 121°C, 20-30
minutes or 134°C, +2 atm overpressure 10 minutes.
Chemical agents used for gas sterilisation? - Correct Answers -Etilene oxide,
formaldehyde, beta-propiolacton
The theoretical background of plasma sterilisation? - Correct Answers -Hydrogen-
peroxide in high electric field will form plasma stage. The produced free radicals will kill
the microbes. At the end of the procedure will be produced water, oxygen and other
nontoxic products.
Biological method used for checking the effectivity of the sterilisation. - Correct Answers
-By Bacillus/Geobacillus stearothermophilus spores. If the procedure was performed in
correct way, the spores cannot be cultivated.
Detection of the presence of pyrogenic material in drugs? - Correct Answers -LAL test;
The blood of the horseshoe crab will coagulate in the presence of the LPS
What are the disinfectants? - Correct Answers -Chemical agents used on
inanimate/non-living surfaces.
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