PLTW Medical Interventions Unit 1 Exam With
Questions And Answers
Medical Intervention - Answer Any measure whose purpose is to improve health or alter
the course of disease
Medical interventions help maintain health and homeostasis in the body.
Pathogen - Answer A specific causative agent of disease
Outbreak - Answer A sudden rise in the incidence of a disease
Patient Symptoms/Risk Factors Organizer - Answer Chart listing patient's symptoms
along with every lifestyle and background factor that puts him or her at risk for specific
diseases.
Primer - Answer Complementary DNA fragment enabling the enzyme to bind to the DNA
for DNA replication.
Bioinformatics - Answer Acquisition, organization, storage, and use of biological data
and information using specially computers in relation to molecular genetics and
genomics.
Genome - Answer The genetic material of an organism
PCR Amplification - Answer Melt: Highest temp, strands separate
Anneal: Lowest temp, primers bind
Extend: Medium temp, DNA replicates (Polymerase)
Final Extension: Ten minutes at medium temp, allow time for completion of DNA
, replication
DNA Sequencing - Answer This is the DNA separated by size (smallest to largest) using
gel electrophoresis or some similar technique. It is color-coded, due to fluorescent
markers on the stoppers of the DNA that indicate which nucleotide it represents. In that
size order, the nucleotides represent the DNA sequence. BLAST can be used to identify
the bacteria.
Solute-Answer A substance dissolved in another substance
Solvent - A substance, normally a liquid, in which another substance is dissolved.
Solution - A homogeneous mixture of two or more substances. They can be in any
physical state.
Concentration - The quantity of a particular substance contained in a unit measure of
another substance
Antibody - An antigen-binding immunoglobulin, produced by B cells, which serves as the
effector in an immune response
Antigen - A foreign macromolecule not from the host that provides the immune system
with a chemical signal identifying it as foreign.
ELISA - Answer In vitro quantitative test for an antibody or antigen, in which the test
material is absorbed onto a surface, and the absorbed material is exposed to either a
complex of an enzyme linked to an antibody specific for the antigen, or to an enzyme
linked to an anti-immunoglobulin specific for the antibody, followed by a reaction of the
enzyme with a substrate to yield a coloured product corresponding to the concentration
of test material.
Steps of ELISA - Answer 1. Sample addition: The sample is added to a plastic well; the
proteins bind to the cell wall. A detergent has washed away unbound proteins and has
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