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MICROBIOLOGY 2420 LAB EXAM 1 STUDY GUIDE
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MICROBIOLOGY 2420 LAB EXAM 1STUDY GUIDE
Resolution
Clarity of an image. Ability to distinguish fine detail using a magnifying
instrument.
Magnification
Enlargement power of an instrument.
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Working distance
Distance between the slide and the tip of the lens.
Numerical aperture
The measure of a lens' ability to "capture" light coming from the
specimen and use it to make the image.
par focal
the ocular lens will not lose focus when the objective lens is adjusted
ocular micrometer
an embedded ruler in one of the eye pieces, with unspecified
graduations
stage micrometer
a type of microscopic slide with a ruler divided in to 10um and 100um
increments
ocular lenses
magnifies and created a virtual image
revolving nose piece
to change objective lenses
objective lenses
produces a magnified real image
arm
connects body to base
stage
Supports the slide being viewed
stage clip (mechanical stage)
holds slide in place
,mechanical stage adjustment knob
to adjust the stage forward, back, and side to side
iris diaphragm lever
regulates the amount of light passing through the condenser
lamp
transmits light
power switch of microscope
turns on/off power to the light source
light intensity knob
adjusts how bright the light is
coarse focus knob
raises and lowers stage to bring specimen in to focus
fine focus knob
brings the image in to sharpest focus
base
supports the microscope
Magnifying capacity of ocular lens
10x
Scanner, magnifying capacity
4x
Low Power, magnifying capacity
10x
High/Dry Power, magnifying capacity
40x
Oil Immersion, magnifying capacity
100x
Scanner, Actual (total) magnification
40x
Low Power, Actual (total) magnification
100x
High/Dry Power, Actual (total) magnification
400x
Oil Immersion, Actual (total) magnification
1000x
These activities are done in focusing specimen under the oil immersion
objective lens:
a. put a drop of immersion oil on the surface of the slide
b. focus specimen under LPO
, c. remove oil from the lens
d. focus the specimen under OIO with fine focus knob
e. lower the stage and remove slide
What is the best correct sequence of the order of these activities
b-a-d-e-c
What must be used to remove immersion oil from the oil immersion
objective?
lens paper
Comparing the size of the object viewed under the low power objective
(LPO) with that viewed under oil immersion objective (OIO) , how many
times bigger is size of the image of the object in the OIO?
10x
Which of these statements is incorrect regarding microscopy?
A - Observing specimens at higher magnification requires higher amount
of light.
B - The scanner has the lowest numerical aperture.
C - The oil immersion objective has the longest working distance.
D - When a specimen is focused under the scanner, it is possible to see
the same specimen after switching objective lens. This is because the
microscope is said to be parfocal.
C
Which parts of the microscope are involved in the magnification or
enlargement of the specimen?
-objective lenses
-ocular lenses
In the eukaryotic microorganisms observed in this laboratory exercise,
which one is an alga?
-Saccharomyces cerevisae
-Spyrogira
-Amoeba proteus
-Penicillum
Spyrogira
The estimated actual size of a microscopic organism such as
the Paramecium caudatum, remains the same when observed under the
scanner and in the oil immersion objective. Only the magnification of the
specimen differs.
True
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