In this capsule between your hands, we have done hard and precise work to summarise the most important notes on practical microbiology (for undergraduate medical students). We hope you all guys find this capsule helpful. - - - - This capsule consists of 4 pages.
Culture Media
Medium Color Organism Growth
White / Yellow Simple medium
Nutrient agar Yellow Staph aureus Yellow endopigments
Green Pseudomonas aeruginosa Green exopigments
Swarming Proteus Swarming motility
Pink / yellow Staphylococci Mannitol fermentation
Mannitol salt agar Yellow Staph aureus Fermenter
Pink Staph epidermidis Non fermenter
Red Enriched medium Alpha / Beta / Gamma
Alpha Green Pneumococci – Viridans Partial hemolysis
Blood agar streptococci
Beta Yellow Strept pyogenes – Strept Complete hemolysis
(Clear zone) agalactiae – staph aureus
Gamma White No hemolysis
Chocolate agar Brown Neisseria – Hemophilus Heated Blood
Loffler’s serum White (Tube) C. diphtheria Enriched medium
Lowenstein Jensen (LJ) Green M. tuberculosis Selective and enriched
Pink / yellow Enterobacteriaceae
MacConkey agar Pink E. coli / Klebsiella Lactose fermenters
Yellow Salmonella / shigella / proteus Lactose Non fermenters
Test sugar fermentation
Red Gram negative bacilli (Glucose-Lactose-Sucrose)
and H2S production.
Triple sugar iron (TSI) Red / Red P. aeruginosa No fermentation
Red / Yellow Salmonella / Shigella Ferment glucose
E. coli / Klebsiella With GAS
Yellow / Yellow Cracking. Ferment all sugars
V. cholera No GAS.
Red / Black Salmonella / Proteus Produce H2S
TCBS agar Green For vibrio Selective medium
Yellow V. cholerae Ferment sucrose
Cooked meat broth Meat inside a tube Anaerobic bacteria Anaerobic medium
Anaerobic Jar Anaerobic bacteria Anaerobic medium
(Gaspack)
Tissue culture bottles For Viruses Cultivation
SDA agar Candida Creamy White Candida Selective for Fungi
Tetrathionate Yellow
Enrichment medium for salmonella
Selenite broth White
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