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Csmls Histology Questions And Answers With Verified Solutions Already Passed!!!
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Csmls Histology Questions And Answers With Verified Solutions Already Passed!!!
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Csmls Histology Questions AndAnswers With Verified Solutions Already
Passed!!!
3 types of Carbohydrates demonstrated in tissues - ANSWER✔✔ Glycoproteins,
polysaccharides, proteoglycans
What stain is used for glycogen? - ANSWER✔✔ PAS - because glycogen has
vicin diols
Where is glycogen found in the body? - ANSWER✔✔ Liver, skeletel and cardiac
muscle, hair follicles, vaginal and ectocervical epithelium, mesothelium cells,
neutrophils and megakaryocytes.
What accounts for the staining reaction of Glycoproteins? - ANSWER✔✔ sialic
acid goups and vicin diols (PAS), also contains carboxyl acid group (Alcian Blue).
Where are glycoproteins in the body? - ANSWER✔✔ Most are associated with
epithelial tissues. They are classified as either neutral or acidic depending on
whether they contain a Sulphate or Carboxylate residues.
What do neutral glycoproteins lack? Where do you see them? - ANSWER✔✔
They lack bother Carboxylate (COOH) and Sulphate (SO3H). They are found in
goblet cells in the stomach as well as paneth cell granules in the small bowel.
Where are acidic glycoproteins that contain only COOH found in the body? -
ANSWER✔✔ They can be found submaxillary gland, small intestine, fetal
extracellular matrix, upper part of colonic crypts, sublingual gland.
, Where are acidic glycoproteins that contain both COOH and SO3H found in the
body? - ANSWER✔✔ Mainly found in colonic goblet cells.
What are Proteoglycans (Glycosaminoglycans)? - ANSWER✔✔ They are large,
unbranched carbohydrates with small poly peptide chains.
Where in the body can you find Proteoglycans? - ANSWER✔✔ Connective tissues
such as cartilage, bone, tendons, ect. Referred to as acid or neutral mucins.
What compound reacts with Cationic dyes in a Carbohydrate? - ANSWER✔✔ The
acid containing sugar (COOH or SO3H)
What are the routine stains for Carbohydrates ? - ANSWER✔✔ PAS, Alcian Blue
Muricarmine, Colloidal iron, Metachromasia, Lectins
2 main steps in PAS method? - ANSWER✔✔ Oxidize carbohs with periodic acid
to create aldehydes.
Demonstrating the resulting aldehydes with Schiffs reagent.
Potential false PAS positives ? - ANSWER✔✔ When glutaraldehyde is used as a
fixative, and only some lipids found in frozen sections.
What does Schiffs reagent do in PAS? - ANSWER✔✔ It is attracted to an reacts
with aldehydes.
Some Proteoglycans contain vicin diols but do not stain with PAS, why ? -
ANSWER✔✔ Because the periodate ion is repelled by the COO and SO3,
therefore oxidation does not occur and the aldehydes are not exposed.
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