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CSMLS Heme Review Questions And Answers With Verified Solutions Already Passed!!!
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CSMLS Heme Review Questions And Answers With Verified Solutions Already Passed!!!
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CSMLS Heme Review Questions AndAnswers With Verified Solutions Already
Passed!!!
Manual cell count calculation - ANSWER✔✔ Average # of cells counted x
dilution factor
# of squares x Area of squares (LxW mm2)x Depth (0.1mm)
Then convert mm3 to x 109/ L - divide by 1000
Manual cell count sources of error: overfilling chamber - ANSWER✔✔ - falsely
low count
- cells get pushed out of grid
- cells lost in moat
Manual cell count sources of error: angle of pipette - ANSWER✔✔ - must be
vertical
- angle will push cells out of grid
- falsely low count
Manual cell count sources of error: evaporation - ANSWER✔✔ - chamber sits too
long before count results in lost cells
- falsely low count
Manual cell count sources of error: dirty chamber - ANSWER✔✔ - falsely
elevated
- dirt counted as cells
,RBC indicies: MCV - ANSWER✔✔ HCT X 1000
RBC
RBC indices: MCH - ANSWER✔✔ HGB
RBC
RBC indices: MCHC - ANSWER✔✔ HGB
HCT
NRBC correction calculation - ANSWER✔✔ 100 x automated total WBC/L
100 + NRBCs/ 100 WBCs
- change absolute count my multiplying relative by corrected WBC count
greatly raised Hgb result on CBC - ANSWER✔✔ - check history for recent
transfusion before proceeding
decreased Hgb result on CBC - ANSWER✔✔ - check chemistry results for
suspected anemias
true hemorrhage vs bloody tap (CSF) - ANSWER✔✔ - spin down CSF
- check supernatant: if clear= traumatic tap, or very recent hemorrhage (becomes
xanthochromic after 36 hrs)
- if xanthochromic: true hemorrhage
,cytospin - ANSWER✔✔ - concentrates cells for adequate analysis (body fluids)
what can you add to specimen to prevent cell smudging in body fluid analysis -
ANSWER✔✔ - albumin
- prevents cells from being smudged or washed off
- PBF doesn't usually need addition of albumin due to proteins in whole blood
What component of the sickle reagent causes RBCs to form tactoid crystals? -
ANSWER✔✔ sodium metabisulphite
- slide test
Most common deficiency in the Embden-Mayerhof pathway - ANSWER✔✔ PK
deficiency
Cause of false (+) stomatocytosis - ANSWER✔✔ - humidity introduced to slide
- stomatocytes a result of abnormal erythrocyte hydration
Reasons for MCHC to be > 360 - ANSWER✔✔ - spherocytes
- agglutination
- lipemia
- hemolysis
- icterus
increased MCV - ANSWER✔✔ macrocytes
, decreased MCV - ANSWER✔✔ microcytes
decreased MCH, MCHC - ANSWER✔✔ - RBCs have less hgb
- increased central pallor
- hypochromasia
decreased HGB, MCH, MCHC - ANSWER✔✔ - abnormal production
- anemias
MCV normal, increased RDW - ANSWER✔✔ - dual pop
- variation in cell size
- possible recent transfusion
Under-filling the EDTA blood collection tube - ANSWER✔✔ - increased
anticoagulant
- Erroneous low blood cell counts & hematocrits
- Morphologic changes to RBCs and WBCs
- Staining alteration
- Excess EDTA can shrink red cells
Interstitial fluid from a skin puncture or the improper flushing of a catheter causes
_______________ Hct readings - ANSWER✔✔ decreased
Blood smears should be prepared within __________ hours of collection -
ANSWER✔✔ two
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