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FSC100 Exam review questions with correct answers (updated)

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FSC100 Exam review questions with correct answers (updated)

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  • September 30, 2024
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  • 2024/2025
  • Exam (elaborations)
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  • FSC100
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FSC100 Exam review

Forensic biology incorporates related areas of - correct answer ✔✔- Bodily fluid identification (serology)

- Deoxyribonucleic acid (DNA) analysis



Serology - correct answer ✔✔-Techniques are used to screen evidence items for the prescience and type
of body fluid disposition

- analyses and targets a the biochemicals and cells that define distinctive functional characteristics of
different body fluids: hemoglobin in blood and spermatozoa in semen



Deoxyribonucleic acid - correct answer ✔✔genetic information that dictates the firm and development
of an organism. A molecule found in almost all human cells



DNA is the genetic blueprint of: - correct answer ✔✔Life



Each strand of the helix is composed of: - correct answer ✔✔long chains of nucleotides, composedof a
phosphate group, a type of sugar called deoyribose, and one of four nitrogen-containing bases



4 bases of DNA - correct answer ✔✔Adenine(A), Thymine(T), Guanine(G), Cytosine(C)`



What 2 subunits form base pairs(BP) - correct answer ✔✔- Adenine(A) with Thymine(T)

- Guanine(G) with Cytosine(C)



Strands of DNA form codes of: - correct answer ✔✔proteins (genes) that provide genetic information in
the organism, like hair color, height, hair texture and skin



Human DNA contain approx.: - correct answer ✔✔3 billion total number of base pairs



Chromosomes - correct answer ✔✔structures carrying molecular strands of DNA

,Genome - correct answer ✔✔one cell collection of chromosomes



Humans inherit: - correct answer ✔✔a genome of 23 chromosomes from each parent for a total of 46.



Rosalind Franklin - correct answer ✔✔- died at 36

- contributions to understanding DNA, including RNA viruses coal and graphite

- found being female is significant as oppurtunities were limited to women chemical structure of DNA
similarly to all cellular organisms.



First DNA case - correct answer ✔✔- Narborough England

- rape and murder of 2, 15yo girls

- in ealry 1980, Dr Jeffery's lab produced first "restricition fragment Length polymorphism" = RFLP



Dr Alec Jeffery - correct answer ✔✔- Studied at Leicester University - England

- studying DNA variations and the evolution of gene families

- his interest and goal was to develop markers (a type of identification tag) to track the position of these
genes



RFLP - correct answer ✔✔Restriction Fragment Length Polymorphism

- Created 1980 in Dr. Jeffery's lab

- a molecular method of genetic analysis that allows individuals to be identified based on unique
patterns of restriction enzyme cutting in specific regions of DNA



1984 - correct answer ✔✔Jefferys developed and used the first DNA tests to be applied to a criminal
case



DNA Fingerprinting - correct answer ✔✔- first-generation typing system made by Dr.Jefferys.

- this method looks at sections of DNA located in different regions on different chromosomes

- multilocus typing system

, DNA typing - correct answer ✔✔describes the system of assessing variations in sections of DNA for
analysis



DNA profiling - correct answer ✔✔the second generation typing system (a more recent development)
that looks at one area- a single locus- on a single chromosome. this is the method now in use for forensic
analysis



DNA profile - correct answer ✔✔most simply viewed as a set of numbers representing the DNA 'variants'
as target locations



DNA locations - correct answer ✔✔also known as 'Loci' and are the genetic equivalents of addresses or
postal codes



genetic differences at loci are: - correct answer ✔✔variants or alleles



STR analysis - correct answer ✔✔a method of DNA profiling that compares the lengths of STR sequences
at specific sites in the genome



Regions with repeats of nine to eighty base pairs are referred to as: - correct answer ✔✔variable
number tandem repeats



Regions containing 2 to 5 base pair repeats are: - correct answer ✔✔short tandem repeats



today robotics use ____________ technologies that openbiological cells, selectively bind DNA to
magnetic beads, and then wash off the non-DNA components, leaving the purified DNA for further
analysis - correct answer ✔✔solid-phase



DNA standards and protocols - correct answer ✔✔- introduced in 1988

- arguments by scientists over practical applications and statistical calculations

- 1990s saw rapid increase in acceptance and usage

- by 1994, typing was firmly established from use in forensic investigations

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