MB (ASCP) Exam Questions With Verified
Answers.
Predominant form of DNA - answerRight-handed B
DNA and histone interaction is due to what type of bond? - answerIonic
What stains to the minor groove of DNA duplex? - answerSYBR Green
What is the most abundant type of RNA? - answerRibosomal RNA (...
DNA and histone interaction is due to what type of bond? - answer✔Ionic
What stains to the minor groove of DNA duplex? - answer✔SYBR Green
What is the most abundant type of RNA? - answer✔Ribosomal RNA (rRNA)
What does RNA act as in a retrovirus? - answer✔Genome and mRNA
What increases half-life of mRNA? - answer✔5' Methyl Cap
What kind of DNA is most likely to be transcriptionally active? - answer✔Euchromatin
What is the most common type of eukaryotic DNA? - answer✔Satellite DNA
What specimen has the most DNA according to molecular weight? - answer✔White Blood Cells
What amino acids compose histones? - answer✔Lysine and Arginine
Amino acid that all proteins start with: - answer✔Methionine
What protects the DNA strand during replication? - answer✔Single-stranded binding protein
Leading Strand - answer✔3' to 5' template
Lagging Strand - answer✔5' to 3' template
What DNA strand is being copied in transcription? - answer✔Antisense Strand
First step after newly formed mRNA is made? - answer✔Addition of poly(A) tail
Every time the chromosome is copied it would become shorter if not for what enzyme? -
answer✔Telomerase
What polymerase has repair activity? - answer✔Beta/Epsilon
Which PCR requires two steps for specificity? - answer✔Nested PCR
Which microorganism is detected by branched DNA amplification? - answer✔DNA or RNA
Which technique uses RNA, antibodies, and DNA? - answer✔Hybrid Capture Assay
Use of bisulfite treatment of DNA to determine its pattern of methylation - answer✔Bisulfite
DNA
What tests are isothermal? - answer✔NASBA, TMA, & SDA
Which leukemia is associated with FLT3 gene? - answer✔AML
T-cell gene rearrangement is seen in what malignancy? - answer✔Sezary Syndrome
Which translocation causes Acute Premyelocytic Leukemia? - answer✔t 15;17
Ewing Sarcoma - answer✔t 11;22
Synovial Sarcoma - answer✔t X;18
Follicular Lymphoma - answer✔t 14;18
What genes would be screened in a colorectal panel? - answer✔MSH2 & MLH1
What gene mutation causes a high risk of breast or ovarian cancer? - answer✔BRCA1 and
BRCA2
What does MSI test for? - answer✔Mutations in mismatch repair genes (MMR)
What disease is related to factor V coagulation gene (F5 1q23)? - answer✔Thrombossis
How many copies are in a full Fragile X mutation? - answer✔200-2000+ CGG repeats
What disease is known for numerous mutations and large deletions? - answer✔Muscular
Dystrophy (Duchenne)
What HPV causes cervical cancer? - answer✔16 & 18
You receive a paraffin embedded specimen for HPV typing, which method should you use? -
answer✔PCR
Significant change in HIV viral load: - answer✔0.5 log10
Lowest viral concentration that can be detected over background or a negative control in the test
assay - answer✔Limit of Detection (LOD)
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