The correct operational relationship E. When pH = pKa , the ionizable compound in question (whether acid or base) will be
between pKa and pH is that: half protonated and half deprotonated.
A. both are log functions.
B. both are always <7 for acids, and >7
for bases.
C. These two concepts are not
operationally related in any way since
biological fluids contains mixtures of too
many acids and bases.
D. When pH = pKa , the compound in
question will have a charge of +0.5.
E. When pH = pKa , the ionizable
compound in question (whether acid or
base) will be half protonated and half
deprotonated.
A weak acid HA has a pKa of 4.11. Which D. Higher [A-] and low [HA]
of the following is true for a solution of
HA at pH 7.11? My Note:
A. Very high [HA] and essentially no [A-] pH > pKa = deprotonated
B. Higher [HA] and low [A-] pH < pKa = protonated
C. Equal [HA] and [A-]
D. Higher [A-] and low [HA]
A weak acid HA has a pKa of 7.39. Which C. Equal [HA] and [A-]
of the following is true for a solution of
HA at pH 7.39?
A. Very high [HA] and essentially no [A-]
B. Higher [HA] and low [A-]
C. Equal [HA] and [A-]
D. Higher [A-] and low [HA]
,A weak acid HA has a pKa of 6.78. Which B. Higher [HA] and low [A-]
of the following is true for a solution of
HA at pH 5.22? My Note:
A. Very high [HA] and essentially no [A-] pH > pKa = deprotonated
B. Higher [HA] and low [A-] pH < pKa = protonated
C. Equal [HA] and [A-]
D. Higher [A-] and low [HA]
a. Give the one letter and 3 letter code for Lys (K), Arg (R), His (H); exterior
the basic amino acids.
b. Would you expect these amino acids to
be on the interior or exterior of a
globular protein?
Glutamic acid is one of 2 acidic amino Asp (D); negative 1
acids.
a. What is the one letter and 3 letter code
for the other one?
b. What is the charge of the side chains
of these 2 amino acids?
Interior
Would you expect Leu to be in the
interior or exterior of a globular protein?
My Note: Leucine is NON-POLAR
Give the one letter and three letter code Ser (S), Thr (T), Tyr (Y)
for the 3 amino acids that have side
chains that can get phosphorylated.
Which amino acid can react with itself to Cys (C)
form a dimer by formation of a disulfide
bond?
At a pH above its pKa, the phenol group of tyrosine is DEPROTONATED.
At a pH above its pKa, the phenol group
of tyrosine is ______.
My Note:
A. protonated
pH > pKa = deprotonated
B. deprotonated
pH < pKa = protonated
At a pH below its pKa, the side chain-amino group of lysine is PROTONATED.
At a pH below its pKa, the side chain-
amino group of lysine is ______.
My Note:
A. protonated
pH > pKa = deprotonated
B. deprotonated
pH < pKa = protonated
Which of the following amino acids has a E) Glu
charged polar side chain at pH 7.0? What Charge is negative 1
is the charge?
A) Leu
B) Ala
C) Met
D) Trp
E) Glu
E) Asn
Which of the following amino acids does
not have an ionizable side chain?
A) Asp B) Cys C) Lys D) His E) Asn
, Which of the following amino acids has B) Thr
an uncharged polar side chain at pH 7.0?
A) Arg
B) Thr
C) Glu
D) Pro
E) Ile
Which of the following tripeptides would C) GYA
be expected to be the most hydrophobic?
A) KYG
B) KYA
C) GYA
D) DYA
E) DYG
C) glutamine synthetase (621 kDa)
You are trying to separate five proteins, B) RNA polymerase (99 kDa)
which are listed below, by gel filtration E) hemoglobin (62 kDa)
chromatography. Rank the proteins from D) interferon-γ (34 kDa)
first to last to elute. A) cytochrome c (12 kDa)
A) cytochrome c (12 kDa)
B) RNA polymerase (99 kDa) C, B, E, D, A
C) glutamine synthetase (621 kDa)
D) interferon-γ (34 kDa) My Note: in gel filtration chromatography, biggest molecules elute first, smallest
E) hemoglobin (62 kDa) molecules elute last (because the small ones get stuck in the beads so they can't elute
as quickly).
SDS-PAGE separates proteins primarily B) MASS
due to differences in
A) isoelectric point.
B) mass.
C) polarity.
D) solubility.
E) amino acid sequence.
Which statement is true concerning the E. All statements are true
structure of proteins?
A. The primary structure is the sequence
of amino acids.
B. Alpha helices and beta sheets are
examples of secondary structure.
C. Side chains (R-groups) of amino acids
can be hydrophilic or hydrophobic.
D. Proteins made of two or more
polypeptide chains have quaternary
structure.
E. All statements are true.
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