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BMD 410 Lecture 5 Questions and Correct Answers | Latest Update $9.99   Add to cart

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BMD 410 Lecture 5 Questions and Correct Answers | Latest Update

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  • BMD 410

What is the proteome?  functional representation of the genome -entire set of proteins expressed and modified by a cell under a particular set of biochemical conditions. -not all proteins are expressed in every cell How can we analyze the proteome?  Isolate, characterize and catalog th...

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  • October 3, 2024
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  • Exam (elaborations)
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  • BMD 410
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Best Grades | Must Pass | Latest Update | Correct Answers | 2024/ 2025


BMD 410 Lecture 5 Questions and Correct
Answers | Latest Update
What is the proteome?


 functional representation of the genome




-entire set of proteins expressed and modified by a cell under a particular set of biochemical

conditions.




-not all proteins are expressed in every cell




How can we analyze the proteome?


 Isolate, characterize and catalog the proteins




Characterizing proteins


 1. Get access (bust open cell)

2. Purify sample


3. Assess the purity of the sample


4. Determine structure by NMR or X-ray crystallography




What is the purpose of cell lysis


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, Best Grades | Must Pass | Latest Update | Correct Answers | 2024/ 2025

 Disrupt the cell membrane (yields a homogenate, a mixture of the cell

contents, but not bound by any membrane)




Why/how is differential centrifugation used?


 1. Centrifuged at low speeds (heavy pellet forms at bottom, light

supernatant at top)

2. Pellet can be centrifuged again to yield another pellet




-used to separate proteins




What is water of solvation?


 -Adding salt to solution will bring protein out of solution

-the Na and Cl ions interact with water and decrease the number of stabilizing hydrogen

bones between water and protein


-Salting out can be used to isolate proteins of interest from different combination of proteins.




Salt can be removed by


 dialysis where large proteins will retain in bag and can't move through

the membrane while ions move out




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