What is the proteome?
functional representation of the genome
-entire set of proteins expressed and modified by a cell under a particular set of biochemical
conditions.
-not all proteins are expressed in every cell
How can we analyze the proteome?
Isolate, characterize and catalog th...
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BMD 410 Lecture 5 Questions and Correct
Answers | Latest Update
What is the proteome?
functional representation of the genome
-entire set of proteins expressed and modified by a cell under a particular set of biochemical
conditions.
-not all proteins are expressed in every cell
How can we analyze the proteome?
Isolate, characterize and catalog the proteins
Characterizing proteins
1. Get access (bust open cell)
2. Purify sample
3. Assess the purity of the sample
4. Determine structure by NMR or X-ray crystallography
What is the purpose of cell lysis
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, Best Grades | Must Pass | Latest Update | Correct Answers | 2024/ 2025
Disrupt the cell membrane (yields a homogenate, a mixture of the cell
contents, but not bound by any membrane)
Why/how is differential centrifugation used?
1. Centrifuged at low speeds (heavy pellet forms at bottom, light
supernatant at top)
2. Pellet can be centrifuged again to yield another pellet
-used to separate proteins
What is water of solvation?
-Adding salt to solution will bring protein out of solution
-the Na and Cl ions interact with water and decrease the number of stabilizing hydrogen
bones between water and protein
-Salting out can be used to isolate proteins of interest from different combination of proteins.
Salt can be removed by
dialysis where large proteins will retain in bag and can't move through
the membrane while ions move out
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