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BIO-205L Midterm Exam Review Guide Questions And Answers 2024/2025 Update
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BIO-205L Midterm Exam Review Guide Questions And Answers 2024/2025 Update
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BIO-205L Midterm Exam ReviewGuide Questions And Answers
2024/2025 Update
Lab safety protocol ANS✔✔ - Safety goggles
- Lab coat
- Close toed shoes
- Hair back
- Wash hands before and after class
- gloves
- Clean station before and after class
- eating, drinking, and smoking are NOT allowed
- no sleeveless tops/ thighs should be covered
- must know all exits of the lab
- put away all staining reagents IMMEDIATELY after use
Nosocomial infections and their role in the spread of contagious diseases ANS✔✔ - Is a disease acquired
from a hospital setting
- Are infections that develop as a result of a stay in the hospital or are produced by microorganisms and
viruses acquired during hospitalization; most common are Staphylococcus aureus, MRSA, Enterococci,
VRE
Using agar slants for long-term storage of microbial cultures ANS✔✔ - Slants have screw-cap that
prevents the agar from drying out
, - The tube can be tightly capped for relatively long-term storage of an isolate with low risk of
contamination
Using broth (liquid medium) tubes for growing microorganisms and generating cultures for experimental
use ANS✔✔ - in broth a species may display motility and/or a characteristic pattern of association
among individual cells, such as chains or clusters, that is not as obvious in agar
Microbe ANS✔✔ - A microorganism, especially a bacterium causing disease or fermentation
Bacterial media ANS✔✔ Allows us to isolate bacterium, identify morphology
Bacterial colony ANS✔✔ - Is a visible mass of microorganisms all produced by a single cell
Bacterial culture ANS✔✔ - Is a method of multiplying microbial organisms by letting them reproduce in
predetermined culture medium under controlled laboratory conditions.
Contamination ANS✔✔ - Accidental introduction of infectious material
Sterilization ANS✔✔ The process that completely destroys all microbial life, including spores.
- Utilizing equipment such as a spirit lamp, bactincinerator, and an autoclave, we can sterilize and reuse
Aseptic methods ANS✔✔ In order to prevent contaminating microbes from entering cultures, we use
aseptic methods to prevent such. The streak for isolation technique is the most common aseptic
technique used to obtain discrete colonies of bacteria given in a mixed broth
Hand washing: Semmelweis ANS✔✔ wash your hands constantly: before using equipment/putting on
gloves, always after coming in contact with specimens, after taking off gloves, and before leaving class
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