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uta nursing microbiology lab 2 Test | Quizzes with Answers Verified 100% Correct
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uta nursing microbiology lab 2 Test | Quizzes with Answers Verified 100% Correct
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uta nursing microbiology lab 2 Test | Quizzeswith Answers Verified 100% Correct
semi-solids - ✔✔have a lower concentration of solidifying agent (such as agar)
that allow them to be less firm but not a liquid.
undefined media (complex media) - ✔✔the exact ingredients concentrations
are unknown.
defined media - ✔✔all ingredients are purified and known when added to
prepare the media.
Sterilization - ✔✔a process that eliminates or deactivates all biological agents
(bacteria, fungi, viruses, etc.).
heat sterilization - ✔✔the most commonly used method when preparing and
sterilizing growth media.
can be performed either through the use of an autoclave or heating the media to
its boiling point.
autoclave parts - ✔✔chamber
jacket
chamber - ✔✔where items are loaded (such as glassware, media, etc.)
, jacket - ✔✔refers to the area surrounding the chamber but within the sealed
vessel of the autoclave.
How an autoclave works - ✔✔When in use, the vessel is sealed off and air is
replaced by steam until the correct temperature and pressure are met (121°C and
15 pounds per square inch). Raising the pressure in the autoclave allows for
liquids to be heated above 100°C while not boiling over. This also allows for the
kill of heat resistant microbes or bacterial endospores.
phases of an autoclave cycle - ✔✔purge phase
sterilization phase
exhaust phase
purge phase - ✔✔steam flows into the chamber and replaces air with steam.
Pressure is ramped up slowly.
sterilization phase - ✔✔The exhaust valve is closed causing the temperature
and pressure to reach the desired setpoint. The autoclave program maintains
these settings for the desired time set.
exhaust phase - ✔✔Pressure is slowly release from the chamber through an
exhaust valve until ambient pressure is reached.
Sterilization - ✔✔the complete removal or killing of all vegetative cells,
endospores, and viruses from the targeted item or environment.
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