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Micro UTA Lab Practical 1 Guide Verified 100% Correct
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Micro UTA Lab Practical 1 Guide Verified 100% Correct
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Micro UTA Lab Practical 1 Guide Verified 100%Correct
CFU - ✔✔colony forming units (estimated number of variable bacteria or fungal cells in a
sample)
TFTC - ✔✔too few to count (less than 30)
TNTC - ✔✔too numerous to count (more than 300)
spectrophotometer - ✔✔measures the cell density of turbidity of a sample as a function of
absorbance (used to determine bacterial cell numbers)
more - ✔✔the more turbid the culture, the ____________ bacteria are present
directly proportional - ✔✔absorbance is ________________________ to cell number
standard plate count - ✔✔Commonly used method to estimate the number of microbes in a
sample. Allows you to count only living cells versus viewing under microscope
dilution - ✔✔necessary to reduce the number in a sample to a level that can be counted
why standard plate count is used - ✔✔to find the concentration of bacteria in the sample
being tested
less - ✔✔when using a spectrophotometer, the more cells there are in a sample, the
________________ light passes through the sample
, small size
lack of contrast - ✔✔factors affecting our ability to see and characterize bacterial cells
basic stains - ✔✔staining solution with a positively charged chromophore, stain the cell
acidic stains - ✔✔staining solution with a negatively charged chromophore, stain the
background
simple staining - ✔✔the use of a single stain to color a bacterial cell
cell shape and arrangement - ✔✔information from simple staining
differential staining - ✔✔allows distinction between different classes of bacteria
ex. gram stain, acid fast stain
Gram stain
acid fast stain - ✔✔examples of differential stains
structural stains - ✔✔designed to stain a particular structural component of a cell
endospore stain
flagella stain - ✔✔examples of structural stains
direct stains - ✔✔procedures that stain cells
negative staining - ✔✔staining the background instead of the cell
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