MCB 2050 Midterm Exam Questions and Answers 100% Solved
0 view 0 purchase
Course
MCB 2050
Institution
MCB 2050
MCB 2050 Midterm Exam Questions and
Answers 100% Solved
1) What are restriction endonucleases?
2) What do they do (or cut) specificically?
3) What happens to the ends of DNA cut by restriction enzymes? - 1)
enzymes that cut at specific sequences (restriction sites) within DNA
molecules
2) th...
-small, up to about 10kb (for easy manipulation; maximum insert size
usually approx 15kilobase pairs)
3) -an origin of DNA replication (for amplification in bacterial cells)
-a selectable marker: antibiotic resistance gene
-at least one unique RE cleavage site outside of the origin and resistance
gene (many have multiple cloning sites, MCS)
What are the characteristics of the pBluescript cloning vector? - ✔✔-
bacterial origin of replication (to allow plasmid replication)
-multiple cloning site (MCS) to allow insertion of foreign DNA
-antibiotic resistance gene for selection of bacteria transformed with the
plasmid
-selectable marker (lacZ) to differentiate cells with plasmids containing
inserted foreign DNA from those without (blue/white screening)
-lac promoter to express mRNA (small (3kb) to allow for large insertions)
-all RE sites in the MCS are unique sites in the plasmid
1) What does the bluescript plasmid express?
2) Where is the MCS located?
3) What does insertion of foreign DNA into the MCS do? What is this
called? - ✔✔1) the E.coli lacZ gene (encodes beta-galactosidase)
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller JOSHCLAY. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $10.99. You're not tied to anything after your purchase.
