Exam (elaborations)
BCH EXAM 2 questions with verified answers updated
- Course
- Institution
BCH EXAM 2 questions with verified answers updated
[Show more]
Content preview
BCH Exam 2 QuestionsIn the free energy diagram to the right, which reaction is most likely to occur at the fastest rate? - correct
answer ✔✔c
The main difference between a catalyzed and an uncatalyzed reaction is that _ - correct answer ✔✔The
activation energy of the catalyzed reaction is lo
Which of the following features is NOT associated with a peptide bond? - correct answer ✔✔Hydrolysis
of the peptide bond has a positive ∆G
In a reaction with a negative ∆G and diagramed as E + S ES E + P, the ES complex_________
a) lowers the energy of the transition state.
b) forms a transition state in which the substrate may be subject to strain, distortion and desolvation
c) forms a transition state which has a lower energy than would occur in an un-catalyzed reaction
d) a and b
e) all of the above - correct answer ✔✔all of the above
Under standard conditions (i.e. no "extras"), Aspartate Transcarbamoylase has an apparent KM of 10
mM aspartate.However, this reaction can be regulated by CTP (an end product of the pyrimidine
pathway initiated by thetranscarbamoylase reaction) and ATP (an end product of the purine biosynthetic
pathway). Which of the following is most likely not correct for this reaction? - correct answer ✔✔c) 0.4
mM CTP would shift the apparent KM to 5 mM
6. Which of the following best describes positive cooperativity?
a) Binding of one substrate molecule inhibits the binding of a second substrate
b) Binding of one substrate molecule enhances the binding of a second substrate
c) Competitive inhibition by an inhibitor
d) US Congress - correct answer ✔✔b) Binding of one substrate molecule enhances the binding of a
second substrate
, For the enzyme enhanced reaction, CO2 + H2O <--> H2 CO3 <--> H+ + HCO3- , which of the following
would most likely help increase the rate of catalysis - correct answer ✔✔Zn2+
Based on the chymotrypsin protease inhibitor TPCK (Tosyl-phenylalanyl-chloromethyl-ketone), which of
thefollowing changes would be most likely to create a suicide inhibitor more specific for trypsin? -
correct answer ✔✔c) Change the phenylalanine-like feature to be more like lysine
The critical serine residue in the active site of chymotrypsin is initially ionized because of the following
residue that accepts a proton from the serine's hydroxyl group: - correct answer ✔✔His
For a solution of Cys (C), at what pH would the net charge = 0? - correct answer ✔✔(1.9+8.4)/2 = 5.5
For a solution of Lysine (K), what percentage of the side chain Rgroup would be ionized at pH = 10.5? -
correct answer ✔✔50%
For a short peptide with the sequence "HELP", which of thefollowing would be closest to the overall
charge if this peptide were foundin normal human blood? - correct answer ✔✔-1
Which of the following peptides will bind most strongly to a cation exchange column at pH 7? (hint,
matrix is negative)
a) MLVSFYAGLATTVIGAM
b) DETYLTFWELDELIEDPE
c) KIRGRVLIGRHARRAKTR - correct answer ✔✔c)KIRGRVLIGRHARRAKTR
Which of the following would be the most appropriate unit for a KM ? - correct answer ✔✔moles / liter
If an enzyme can reach the upper limits of catalytic efficiency, it is referred to as ________. - correct
answer ✔✔c) Achieving Kinetic Perfection (kcat / KM = ~108 - 109 s-1 M-1 )
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller Sakayobako30. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $11.49. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
83750 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now